Your browser doesn't support javascript.
loading
Effect of Previous Alkylating Agent Exposure on Follicle Numbers in Cryopreserved Prepubertal and Young Adult Ovarian Tissue after Long-Term Xenografting.
Nurmio, Mirja; Asadi-Azarbaijani, Babak; Hou, Mi; Kiviö, Ronja; Toppari, Jorma; Tinkanen, Helena; Laine, Tiina; Oskam, Irma C; Jahnukainen, Kirsi.
Afiliación
  • Nurmio M; Research Centre for Integrative Physiology and Pharmacology, Institute of Biomedicine, University of Turku, 20520 Turku, Finland.
  • Asadi-Azarbaijani B; Centre for Population Health Research, University of Turku and Turku University Hospital, 20520 Turku, Finland.
  • Hou M; Faculty of Health Studies, VID Specialized University, 0319 Oslo, Norway.
  • Kiviö R; NORDFERTIL Research Lab Stockholm, Department of Women's and Children's Health, Karolinska Institute and University Hospital, 17164 Stockholm, Sweden.
  • Toppari J; Research Centre for Integrative Physiology and Pharmacology, Institute of Biomedicine, University of Turku, 20520 Turku, Finland.
  • Tinkanen H; Centre for Population Health Research, University of Turku and Turku University Hospital, 20520 Turku, Finland.
  • Laine T; Research Centre for Integrative Physiology and Pharmacology, Institute of Biomedicine, University of Turku, 20520 Turku, Finland.
  • Oskam IC; Centre for Population Health Research, University of Turku and Turku University Hospital, 20520 Turku, Finland.
  • Jahnukainen K; Department of Pediatrics, Turku University Hospital, 20520 Turku, Finland.
Cancers (Basel) ; 14(2)2022 Jan 13.
Article en En | MEDLINE | ID: mdl-35053561
PURPOSE AND METHODS: To elucidate whether previous cancer treatment affects graft recovery and follicle numbers, morphology, and development in grafts, cryopreserved ovarian biopsies obtained from 18 cancer patients aged 1-24 years with and without exposure to chemotherapy were xenografted as 1 mm3 fragments to immunodeficient mice for 22 weeks with exogenous stimulation. RESULTS: Graft recovery showed no association with chemotherapy exposure, pubertal stage, or leukemia contamination. Total follicle number per recovered graft varied between 0 and 1031 in the chemotherapy-exposed and between 0 and 502 in the non-chemotherapy-exposed group. Atretic follicles formed the largest proportion of the follicle pool in chemotherapy-exposed grafts. Increased atresia correlated with exposure to alkylating agents (mean ± SD 8866.2 ± 9316.3 mg/m2) but not with anthracyclines, pubertal stage, or leukemia contamination. CONCLUSION: The observation confirms the harmful effects of alkylating agents on ovarian tissue. Therapy at the median cumulative dose of 8866 mg/m2 leads to the decreased quality of cryopreserved ovarian follicles in children and young adults.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Cancers (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Cancers (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Suiza