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Towards Multi-Analyte Detection with Field-Effect Capacitors Modified with Tobacco Mosaic Virus Bioparticles as Enzyme Nanocarriers.
Welden, Melanie; Poghossian, Arshak; Vahidpour, Farnoosh; Wendlandt, Tim; Keusgen, Michael; Wege, Christina; Schöning, Michael J.
Afiliación
  • Welden M; Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, 52428 Jülich, Germany.
  • Poghossian A; Institute of Pharmaceutical Chemistry, Philipps University Marburg, 35032 Marburg, Germany.
  • Vahidpour F; MicroNanoBio, 40479 Düsseldorf, Germany.
  • Wendlandt T; Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, 52428 Jülich, Germany.
  • Keusgen M; Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, 70569 Stuttgart, Germany.
  • Wege C; Institute of Pharmaceutical Chemistry, Philipps University Marburg, 35032 Marburg, Germany.
  • Schöning MJ; Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, 70569 Stuttgart, Germany.
Biosensors (Basel) ; 12(1)2022 Jan 14.
Article en En | MEDLINE | ID: mdl-35049671
Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO2-Ta2O5 layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1-3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta2O5-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus del Mosaico del Tabaco Tipo de estudio: Diagnostic_studies Idioma: En Revista: Biosensors (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus del Mosaico del Tabaco Tipo de estudio: Diagnostic_studies Idioma: En Revista: Biosensors (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza