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Development and Application of a Multiplex Fluorescent PCR for Shigella Detection and Species Identification.
He, Peiyan; Wang, Henghui; Yan, Yong; Zhu, Guoying; Chen, Zhongwen.
Afiliación
  • He P; Jiaxing Center for Disease Control and Prevention, No. 486, Wen Qiao Road, Zhejiang, Jiaxing, 314050, China.
  • Wang H; Jiaxing Center for Disease Control and Prevention, No. 486, Wen Qiao Road, Zhejiang, Jiaxing, 314050, China.
  • Yan Y; Jiaxing Center for Disease Control and Prevention, No. 486, Wen Qiao Road, Zhejiang, Jiaxing, 314050, China.
  • Zhu G; Jiaxing Center for Disease Control and Prevention, No. 486, Wen Qiao Road, Zhejiang, Jiaxing, 314050, China.
  • Chen Z; Jiaxing Center for Disease Control and Prevention, No. 486, Wen Qiao Road, Zhejiang, Jiaxing, 314050, China. jxcdcmicro@126.com.
J Fluoresc ; 32(2): 707-713, 2022 Mar.
Article en En | MEDLINE | ID: mdl-35044573
This study was to develop a multiplex fluorescent PCR for Shigella detection and species identification. Five primer pairs for Shigella detection and species identification were designed by Primer Premier 5.0. The multiplex fluorescent PCR was optimized by varying single parameter while other parameters were maintained. The multiplex fluorescent PCR assay could correctly detect Shigella and identify four Shigella species with a detection limits of 10 pg genomic DNA per reaction. Testing different strains and clinical samples confirmed the sensitivity and specificity of the multiplex fluorescent PCR. The newly developed multiplex fluorescent PCR assay is simple, sensitive and specific for Shigella detection and species identification. It has a potential to be used in routine Shigella detection and species identification in clinical laboratories.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Shigella / Reacción en Cadena de la Polimerasa Multiplex / Fluorescencia Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Humans Idioma: En Revista: J Fluoresc Asunto de la revista: BIOFISICA Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Shigella / Reacción en Cadena de la Polimerasa Multiplex / Fluorescencia Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Humans Idioma: En Revista: J Fluoresc Asunto de la revista: BIOFISICA Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos