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Multiscale X-ray study of Bacillus subtilis biofilms reveals interlinked structural hierarchy and elemental heterogeneity.
Azulay, David N; Spaeker, Oliver; Ghrayeb, Mnar; Wilsch-Bräuninger, Michaela; Scoppola, Ernesto; Burghammer, Manfred; Zizak, Ivo; Bertinetti, Luca; Politi, Yael; Chai, Liraz.
Afiliación
  • Azulay DN; Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
  • Spaeker O; The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
  • Ghrayeb M; Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.
  • Wilsch-Bräuninger M; Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
  • Scoppola E; The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
  • Burghammer M; Max Planck Institute of Molecular Cell Biology and Genetics, 1307 Dresden, Germany.
  • Zizak I; Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.
  • Bertinetti L; European Synchrotron Radiation Facility, Grenoble 38043, France.
  • Politi Y; Department Structure and Dynamics of Energy Materials, Helmholtz-Zentrum Berlin, 12489 Berlin, Germany.
  • Chai L; B CUBE Center for Molecular Bioengineering, Technische Universität Dresden, 01309 Dresden, Germany.
Proc Natl Acad Sci U S A ; 119(4)2022 01 25.
Article en En | MEDLINE | ID: mdl-35042817
Biofilms are multicellular microbial communities that encase themselves in an extracellular matrix (ECM) of secreted biopolymers and attach to surfaces and interfaces. Bacterial biofilms are detrimental in hospital and industrial settings, but they can be beneficial, for example, in agricultural as well as in food technology contexts. An essential property of biofilms that grants them with increased survival relative to planktonic cells is phenotypic heterogeneity, the division of the biofilm population into functionally distinct subgroups of cells. Phenotypic heterogeneity in biofilms can be traced to the cellular level; however, the molecular structures and elemental distribution across whole biofilms, as well as possible linkages between them, remain unexplored. Mapping X-ray diffraction across intact biofilms in time and space, we revealed the dominant structural features in Bacillus subtilis biofilms, stemming from matrix components, spores, and water. By simultaneously following the X-ray fluorescence signal of biofilms and isolated matrix components, we discovered that the ECM preferentially binds calcium ions over other metal ions, specifically, zinc, manganese, and iron. These ions, remaining free to flow below macroscopic wrinkles that act as water channels, eventually accumulate and may possibly lead to sporulation. The possible link between ECM properties, regulation of metal ion distribution, and sporulation across whole, intact biofilms unravels the importance of molecular-level heterogeneity in shaping biofilm physiology and development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus subtilis / Biopelículas Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2022 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus subtilis / Biopelículas Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2022 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos