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RBM20S639G mutation is a high genetic risk factor for premature death through RNA-protein condensates.
Wang, Chunyan; Zhang, Yanghai; Methawasin, Mei; Braz, Camila Urbano; Gao-Hu, Jeffrey; Yang, Betty; Strom, Joshua; Gohlke, Jochen; Hacker, Timothy; Khatib, Hasan; Granzier, Henk; Guo, Wei.
Afiliación
  • Wang C; Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI 53706, USA.
  • Zhang Y; Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI 53706, USA.
  • Methawasin M; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA.
  • Braz CU; Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI 53706, USA.
  • Gao-Hu J; Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI 53706, USA.
  • Yang B; Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI 53706, USA.
  • Strom J; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA.
  • Gohlke J; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA.
  • Hacker T; Division of Cardiovascular Medicine, Department of Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA.
  • Khatib H; Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI 53706, USA.
  • Granzier H; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA.
  • Guo W; Department of Animal and Dairy Sciences, University of Wisconsin, Madison, WI 53706, USA. Electronic address: wguo2@wisc.edu.
J Mol Cell Cardiol ; 165: 115-129, 2022 04.
Article en En | MEDLINE | ID: mdl-35041844
Dilated cardiomyopathy (DCM) is a heritable and genetically heterogenous disease often idiopathic and a leading cause of heart failure with high morbidity and mortality. DCM caused by RNA binding motif protein 20 (RBM20) mutations is diverse and needs a more complete mechanistic understanding. RBM20 mutation S637G (S639G in mice) is linked to severe DCM and early death in human patients. In this study, we generated a RBM20 S639G mutation knock-in (KI) mouse model to validate the function of S639G mutation and examine the underlying mechanisms. KI mice exhibited severe DCM and premature death with a ~ 50% mortality in two months old homozygous (HM) mice. KI mice had enlarged atria and increased ANP and BNP biomarkers. The S639G mutation promoted RBM20 trafficking and ribonucleoprotein (RNP) granules in the sarcoplasm. RNA Seq data revealed differentially expressed and spliced genes were associated with arrhythmia, cardiomyopathy, and sudden death. KI mice also showed a reduction of diastolic stiffness and impaired contractility at both the left ventricular (LV) chamber and cardiomyocyte levels. Our results indicate that the RBM20 S639G mutation leads to RNP granules causing severe heart failure and early death and this finding strengthens the novel concept that RBM20 cardiomyopathy is a RNP granule disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cardiomiopatía Dilatada / Insuficiencia Cardíaca Tipo de estudio: Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: J Mol Cell Cardiol Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cardiomiopatía Dilatada / Insuficiencia Cardíaca Tipo de estudio: Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: J Mol Cell Cardiol Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido