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Novel cinnamon-laden nanofibers as a potential antifungal coating for poly(methyl methacrylate) denture base materials.
Ribeiro, Juliana Silva; Bordini, Ester Alves Ferreira; Pereira, Gabriel Kalil Rocha; Polasani, Rohitha Rao; Squarize, Cristiane Helena; Kantorski, Karla Zanini; Valandro, Luiz Felipe; Bottino, Marco Cícero.
Afiliación
  • Ribeiro JS; Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 N. University (Room 5223), Ann Arbor, MI, 48109, USA.
  • Bordini EAF; Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Pereira GKR; Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 N. University (Room 5223), Ann Arbor, MI, 48109, USA.
  • Polasani RR; Department of Physiology and Pathology, University Estadual Paulista - UNESP, Araraquara, SP, Brazil.
  • Squarize CH; Post-Graduate Program in Oral Science, Faculty of Dentistry, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande Do Sul State, Brazil.
  • Kantorski KZ; Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 N. University (Room 5223), Ann Arbor, MI, 48109, USA.
  • Valandro LF; Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, USA.
  • Bottino MC; Post-Graduate Program in Oral Science, Faculty of Dentistry, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande Do Sul State, Brazil.
Clin Oral Investig ; 26(4): 3697-3706, 2022 Apr.
Article en En | MEDLINE | ID: mdl-35028732
OBJECTIVES: To modify the surface of denture base material by coating it with cinnamon-laden nanofibers to reduce Candida albicans (C. albicans) adhesion and/or proliferation. MATERIALS AND METHODS: Heat-cured poly(methyl methacrylate) (PMMA) specimens were processed and coated, or not, with cinnamon-laden polymeric nanofibers (20 or 40 wt.% of cinnamon relative to the total polymer weight). Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) analyses of the nanofibers were performed. Antifungal activity was assessed through agar diffusion and colony-forming unit (CFU/mL) assays. Representative SEM morphological analysis was carried out to observe the presence/absence of C. albicans on the fibers. Alamar blue assay was used to determine cell toxicity. Analysis of variance and the Tukey's test were used to analyze the data (α = 0.05). RESULTS: SEM imaging revealed nanofibers with adequate (i.e., bead-free) morphological characteristics and uniform microstructure. FTIR confirmed cinnamon incorporation. The cinnamon-laden nanofibers led to growth inhibition of C. albicans. Viable fungal counts support a significant reduction on CFU/mL also directly related to cinnamon concentration (40 wt.%: mean log 6.17 CFU/mL < 20 wt.%: mean log 7.12 CFU/mL), which agrees with the SEM images. Cinnamon-laden nanofibers at 40 wt.% led to increased cell death. CONCLUSIONS: The deposition of 20 wt.% cinnamon-laden nanofibers onto PMMA surfaces led to a significant reduction of the adhesive and/or proliferative ability of C. albicans, while maintaining epithelial cells' viability. CLINICAL RELEVANCE: The high recurrence rates of denture stomatitis are associated with patient non-adherence to treatments and contaminated prostheses use. Here, we provide the non-patients' cooperation sensible method, which possesses antifungal action, hence improving treatment effectiveness.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polimetil Metacrilato / Nanofibras Límite: Humans Idioma: En Revista: Clin Oral Investig Asunto de la revista: ODONTOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polimetil Metacrilato / Nanofibras Límite: Humans Idioma: En Revista: Clin Oral Investig Asunto de la revista: ODONTOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania