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Routine culture and study of adult human brain cells from neurosurgical specimens.
Park, Thomas I-H; Smyth, Leon C D; Aalderink, Miranda; Woolf, Zoe R; Rustenhoven, Justin; Lee, Kevin; Jansson, Deidre; Smith, Amy; Feng, Sheryl; Correia, Jason; Heppner, Peter; Schweder, Patrick; Mee, Edward; Dragunow, Mike.
Afiliación
  • Park TI; Hugh Green Biobank & Department of Pharmacology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Smyth LCD; Neurosurgical Research Unit, Centre for Brain Research, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Aalderink M; Centre for Free Radical Research, Department of Pathology and Biomedical Science, University of Otago Christchurch, Christchurch, New Zealand.
  • Woolf ZR; Hugh Green Biobank & Department of Pharmacology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Rustenhoven J; Hugh Green Biobank & Department of Pharmacology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Lee K; Center for Brain Immunology and Glia (BIG), Washington University, St. Louis, MO, USA.
  • Jansson D; Department of Physiology, Faculty of Medical Science and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Smith A; Department of Psychiatry and Behavioral Sciences, University of Washington School of Medicine VISN 20 Mental Illness Research, Education and Clinical Centre (MIRECC), VA Puget Sound Health Care System, Seattle, WA, USA.
  • Feng S; Hugh Green Biobank & Department of Pharmacology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Correia J; Hugh Green Biobank & Department of Pharmacology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Heppner P; Neurosurgical Research Unit, Centre for Brain Research, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Schweder P; Department of Neurosurgery, Auckland City Hospital, Auckland, New Zealand.
  • Mee E; Neurosurgical Research Unit, Centre for Brain Research, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
  • Dragunow M; Department of Neurosurgery, Auckland City Hospital, Auckland, New Zealand.
Nat Protoc ; 17(2): 190-221, 2022 02.
Article en En | MEDLINE | ID: mdl-35022619
When modeling disease in the laboratory, it is important to use clinically relevant models. Patient-derived human brain cells grown in vitro to study and test potential treatments provide such a model. Here, we present simple, highly reproducible coordinated procedures that can be used to routinely culture most cell types found in the human brain from single neurosurgically excised brain specimens. The cell types that can be cultured include dissociated cultures of neurons, astrocytes, microglia, pericytes and brain endothelial and neural precursor cells, as well as explant cultures of the leptomeninges, cortical slice cultures and brain tumor cells. The initial setup of cultures takes ~2 h, and the cells are ready for further experiments within days to weeks. The resulting cells can be studied as purified or mixed population cultures, slice cultures and explant-derived cultures. This protocol therefore enables the investigation of human brain cells to facilitate translation of neuroscience research to the clinic.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células-Madre Neurales Tipo de estudio: Guideline Idioma: En Revista: Nat Protoc Año: 2022 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células-Madre Neurales Tipo de estudio: Guideline Idioma: En Revista: Nat Protoc Año: 2022 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Reino Unido