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Regioselective SN2-Type Reaction for the Oriented and Irreversible Immobilization of Antibodies to a Glass Surface Assisted by Boronate Formation.
Adak, Avijit K; Huang, Kuan-Ting; Li, Pei-Jhen; Fan, Chen-Yo; Lin, Po-Chiao; Hwang, Kuo-Chu; Lin, Chun-Cheng.
Afiliación
  • Adak AK; Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
  • Huang KT; Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
  • Li PJ; Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
  • Fan CY; Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
  • Lin PC; Department of Chemistry, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
  • Hwang KC; Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
  • Lin CC; Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan.
ACS Appl Bio Mater ; 3(10): 6756-6767, 2020 Oct 19.
Article en En | MEDLINE | ID: mdl-35019340
Antibodies have exquisite specificities for molecular recognition, which have led to their incorporation into array sensors that are crucial for research, diagnostic, and therapeutic applications. Many of these platforms rely heavily on surface-bound reactive groups to covalently tether antibodies to solid substrates; however, this strategy is hindered by a lack of orientation control over antibody immobilization. Here, we report a mild electrophilic phenylsulfonate (tosylate) ester-containing boronic acid affinity ligand for attaching antibodies to glass slides. A high level of antibody coupling located near the Fc region of the boronated antibody complex could be achieved by the proximal nucleophilic amino acid driven substitution reaction at the phenylsulfonate center. This enabled the full-length antibodies to be permanently tethered onto surfaces in an oriented manner. The advantages of this strategy were demonstrated through the individual and multiplex detection of protein and serum biomarkers. This strategy not only confers stability to the immobilized antibodies but also presents a different direction for the irreversible attachment of antibodies to solid supports in an orientation-controlled way.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Appl Bio Mater Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Appl Bio Mater Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos