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Three-dimensional tissue model in direct contact with an on-chip vascular bed enabled by removable membranes.
Kameda, Yoshikazu; Chuaychob, Surachada; Tanaka, Miwa; Liu, Yang; Okada, Ryu; Fujimoto, Kazuya; Nakamura, Takuro; Yokokawa, Ryuji.
Afiliación
  • Kameda Y; Department of Micro Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8540, Japan. yokokawa.ryuji.8c@kyoto-u.ac.jp.
  • Chuaychob S; Department of Micro Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8540, Japan. yokokawa.ryuji.8c@kyoto-u.ac.jp.
  • Tanaka M; Division of Carcinogenesis, The Cancer Institute, Japanese Foundation for Cancer Research, 3-8-31 Ariake, Koto-ku, Tokyo 135-8550, Japan.
  • Liu Y; Department of Micro Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8540, Japan. yokokawa.ryuji.8c@kyoto-u.ac.jp.
  • Okada R; Department of Micro Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8540, Japan. yokokawa.ryuji.8c@kyoto-u.ac.jp.
  • Fujimoto K; Department of Micro Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8540, Japan. yokokawa.ryuji.8c@kyoto-u.ac.jp.
  • Nakamura T; Division of Carcinogenesis, The Cancer Institute, Japanese Foundation for Cancer Research, 3-8-31 Ariake, Koto-ku, Tokyo 135-8550, Japan.
  • Yokokawa R; Department of Micro Engineering, Kyoto University, Kyoto Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8540, Japan. yokokawa.ryuji.8c@kyoto-u.ac.jp.
Lab Chip ; 22(3): 641-651, 2022 02 01.
Article en En | MEDLINE | ID: mdl-35018934
Three-dimensional (3D) tissue culture is a powerful tool for understanding physiological events. However, 3D tissues still have limitations in their size, culture period, and maturity, which are caused by the lack of nutrients and oxygen supply through the vasculature. Here, we propose a new method for culturing a 3D tissue-a spheroid-directly on an 'on-chip vascular bed'. The method can be applied to any 3D tissue because the vascular bed is preformed, so that angiogenic factors from the tissue are not necessary to induce vasculature. The essential component of the assay system is the removable membrane that initially separates the 3D tissue culture well and the microchannel in which a uniform vascular bed is formed, and then allows the tissue to be settled directly onto the vascular bed following its removal. This in vitro system offers a new technique for evaluating the effects of vasculature on 3D tissues.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Lab Chip Asunto de la revista: BIOTECNOLOGIA / QUIMICA Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Lab Chip Asunto de la revista: BIOTECNOLOGIA / QUIMICA Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido