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Bacteria Inspired Internal Standard SERS Substrate for Quantitative Detection.
Liu, Jiawei; Hong, Zilan; Yang, Weimin; Liu, Chen; Lu, Zhicheng; Wu, Long; Foda, Mohamed F; Yang, Zhilin; Han, Heyou; Zhao, Yanli.
Afiliación
  • Liu J; State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
  • Hong Z; Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.
  • Yang W; State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
  • Liu C; Department of Physics, Xiamen University, Xiamen 361005, People's Republic of China.
  • Lu Z; Leibniz Institute of Photonic Technology Jena-Member of the Research Alliance "Leibniz Health Technologies", Jena 07745, Germany.
  • Wu L; Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Jena 07743, Germany.
  • Foda MF; State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
  • Yang Z; State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
  • Han H; State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
  • Zhao Y; Department of Physics, Xiamen University, Xiamen 361005, People's Republic of China.
ACS Appl Bio Mater ; 4(3): 2009-2019, 2021 03 15.
Article en En | MEDLINE | ID: mdl-35014326
Metal-respiring bacteria are frequently used to recycle metal resources by biosynthesizing nanoparticles on its surface in environment treatment. However, further utilization of biogenetic nanoparticles through combining the advantages of both bacteria and nanoparticles is still limited. Herein, biogenetic Au@Ag nanoislands are utilized as the surface-enhanced Raman spectroscopy (SERS) substrate for quantitative detection. Specifically, Au@Ag nanoislands enhance the Raman signal via surface plasmon resonance, while biomolecules (phospholipid, tyrosine, and phenylalanine, etc.) on bacterium serve as an internal standard to eliminate the discrepancy of the target SERS intensity in different hot spots. Gene-controlled biomolecules in bacteria guarantee the reproducibility of this SERS substrate. The generality of this analytical method is demonstrated by determining rhodamine 6G, malachite green, and uric acid. This discovery solves a pervasive problem in SERS analysis through a simple biogenetic nanosystem, which opens up an avenue to address scientific challenges by using versatile organisms from nature.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Materiales Biocompatibles / Shewanella Tipo de estudio: Diagnostic_studies Idioma: En Revista: ACS Appl Bio Mater Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Materiales Biocompatibles / Shewanella Tipo de estudio: Diagnostic_studies Idioma: En Revista: ACS Appl Bio Mater Año: 2021 Tipo del documento: Article Pais de publicación: Estados Unidos