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Inhibitory Effects of Inonotus obliquus Polysaccharide on Inflammatory Response in Toxoplasma gondii-Infected RAW264.7 Macrophages.
Yan, Kexin; Zhou, Hongyuan; Wang, Meng; Li, Haitao; Sang, Rui; Ge, Bingjie; Zhao, Xin; Li, Chunting; Wang, Wei; Zhang, Xuemei.
Afiliación
  • Yan K; Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, China.
  • Zhou H; Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, China.
  • Wang M; Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, China.
  • Li H; Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, China.
  • Sang R; Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Juye Street, Changchun, Jilin 132109, China.
  • Ge B; Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, China.
  • Zhao X; Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, China.
  • Li C; Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, China.
  • Wang W; Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, China.
  • Zhang X; Agricultural College of Yanbian University, Gongyuan Street, Yanji, Jilin 133002, China.
Evid Based Complement Alternat Med ; 2021: 2245496, 2021.
Article en En | MEDLINE | ID: mdl-35003292
Our previous reports have shown that Inonotus obliquus polysaccharide (IOP) has protective effects against Toxoplasma gondii (T. gondii) infection in vivo. The aim of the present research is to explore the in vitro anti-inflammatory effects of IOP and its mechanism in RAW264.7 macrophages infected by T. gondii. In this study, it is indicated that IOP decreased the excessive secretion of inflammatory cytokines tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-1ß (IL-1ß), IL-4, and IL-6 in T. gondii-infected RAW264.7 macrophages. IOP effectively suppressed the mRNA expression of these cytokines and chemokines monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1α (MIP-1α). Moreover, IOP inhibited the phosphorylation of inhibitor kappa B kinase α/ß (IKKα/ß), inhibitor κBα (IκBα), p65 in nuclear factor-kappa B (NF-κB) signaling pathway and p38, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase 1/2 (ERK1/2) in mitogen-activated protein kinases (MAPKs) signaling pathway. Meantime, IOP prevented NF-κB p65 and c-Jun translocation from the cytoplasm to the nucleus. Further, IOP downregulated the protein expression of toll-like receptor 2 (TLR2) and TLR4 in T. gondii-infected RAW264.7 macrophages. The above results suggest that IOP can inhibit the inflammatory response infected with T. gondii via regulating TLR2/TLR4-NF-κB/MAPKs pathways and exerting its anti-T. gondii role in vitro.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Evid Based Complement Alternat Med Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Evid Based Complement Alternat Med Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos