Your browser doesn't support javascript.
loading
Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes.
Kobayashi, Daichi; Sugiura, Yuki; Umemoto, Eiji; Takeda, Akira; Ueta, Hisashi; Hayasaka, Haruko; Matsuzaki, Shinsuke; Katakai, Tomoya; Suematsu, Makoto; Hamachi, Itaru; Yegutkin, Gennady G; Salmi, Marko; Jalkanen, Sirpa; Miyasaka, Masayuki.
Afiliación
  • Kobayashi D; Department of Immunology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
  • Sugiura Y; Department of Pharmacology, Wakayama Medical University, Wakayama, Japan.
  • Umemoto E; Department of Biochemistry, Keio University School of Medicine, Tokyo, Japan.
  • Takeda A; Laboratory of Microbiology and Immunology, University of Shizuoka, Shizuoka, Japan.
  • Ueta H; MediCity Research Laboratory, University of Turku, Turku, Finland.
  • Hayasaka H; Department of Anatomy, School of Medicine, Dokkyo Medical University, Tochigi, Japan.
  • Matsuzaki S; Laboratory of Immune Molecular Function, Faculty of Science and Engineering, Kindai University, Higashi-Osaka, Japan.
  • Katakai T; Department of Pharmacology, Wakayama Medical University, Wakayama, Japan.
  • Suematsu M; Department of Radiological Sciences, Morinomiya University of Medical Sciences, Osaka, Japan.
  • Hamachi I; Department of Immunology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
  • Yegutkin GG; Department of Biochemistry, Keio University School of Medicine, Tokyo, Japan.
  • Salmi M; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan.
  • Jalkanen S; MediCity Research Laboratory, University of Turku, Turku, Finland.
  • Miyasaka M; MediCity Research Laboratory, University of Turku, Turku, Finland.
Front Immunol ; 12: 786595, 2021.
Article en En | MEDLINE | ID: mdl-35003105
Whereas adenosine 5'-triphosphate (ATP) is the major energy source in cells, extracellular ATP (eATP) released from activated/damaged cells is widely thought to represent a potent damage-associated molecular pattern that promotes inflammatory responses. Here, we provide suggestive evidence that eATP is constitutively produced in the uninflamed lymph node (LN) paracortex by naïve T cells responding to C-C chemokine receptor type 7 (CCR7) ligand chemokines. Consistently, eATP was markedly reduced in naïve T cell-depleted LNs, including those of nude mice, CCR7-deficient mice, and mice subjected to the interruption of the afferent lymphatics in local LNs. Stimulation with a CCR7 ligand chemokine, CCL19, induced ATP release from LN cells, which inhibited CCR7-dependent lymphocyte migration in vitro by a mechanism dependent on the purinoreceptor P2X7 (P2X7R), and P2X7R inhibition enhanced T cell retention in LNs in vivo. These results collectively indicate that paracortical eATP is produced by naïve T cells in response to constitutively expressed chemokines, and that eATP negatively regulates CCR7-mediated lymphocyte migration within LNs via a specific subtype of ATP receptor, demonstrating its fine-tuning role in homeostatic cell migration within LNs.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T / Adenosina Trifosfato / Ganglios Linfáticos Límite: Animals Idioma: En Revista: Front Immunol Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T / Adenosina Trifosfato / Ganglios Linfáticos Límite: Animals Idioma: En Revista: Front Immunol Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza