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Anshen-Buxin-Liuwei pill, a Mongolian medicinal formula could alleviate cardiomyocyte hypoxia/reoxygenation injury via mitochondrion pathway.
Huang, Yu-Jia; Tong, Hai-Ying; Huang, Xian-Ju; Xiao, Xin-Cai; Dong, Yue; Iqbal, Muhammad Sarfaraz.
Afiliación
  • Huang YJ; Department of Medicine and Pharmacy, College of Pharmacy, South-Central University for Nationalities, Wuhan, 430070, Hubei, China.
  • Tong HY; School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, China. haiyingtong@sohu.com.
  • Huang XJ; Department of Medicine and Pharmacy, College of Pharmacy, South-Central University for Nationalities, Wuhan, 430070, Hubei, China. xianju@mail.scuec.edu.cn.
  • Xiao XC; Department of Medicine and Pharmacy, College of Pharmacy, South-Central University for Nationalities, Wuhan, 430070, Hubei, China.
  • Dong Y; Department of Medicine and Pharmacy, College of Pharmacy, South-Central University for Nationalities, Wuhan, 430070, Hubei, China.
  • Iqbal MS; Department of Biotechnology, University of Okara, Okara, Pakistan.
Mol Biol Rep ; 49(2): 885-894, 2022 Feb.
Article en En | MEDLINE | ID: mdl-35001248
BACKGROUND: Anshen Buxin Liuwei pill (ABLP) is a Mongolian medicinal formula that is composed of six medicinal materials: the Mongolian medicine Bos taurus domesticus Gmelin, Choerospondias axillaris (Roxb.) Burtt et Hill, Myristica fragrans Houtt., Eugenia caryophµllata Thunb., Aucklandia lappa Decne., and Liqui dambar formosana Hance. ABLP is considered to have a therapeutic effect on symptoms such as coronary heart disease, angina pectoris, arrhythmia, depression and irritability, palpitation, and shortness of breath. METHODS: H9c2 cardiomyocytes were used to construct a hypoxia/reoxygenation (HR) injury model. CCK-8 assay and Annexin V-FITC cell apoptosis assays were used for cell viability and cell apoptosis determination. The LDH, SOD, MDA, CAT, CK, GSH-Px, Na+-K+-ATPase, and Ca2+-ATPase activities in cells were determined to assess the protective effects of ABLP. The mRNA levels of Sirtuin3 (Sirt3) and Cytochrome C (Cytc) in H9c2 cells were determined by quantitative real-time PCR. RESULTS: The results indicate that HR-treated cells began to shrink from the spindle in an irregular shape with some floated in the medium. By increasing the therapeutic dose of ABLP (5, 25, and 50 µg/mL), the cells gradually reconverted in a concentration-dependent manner. The release of CK in HR-treated cells was significantly increased, indicating that ABLP exerts a protective effect in H9c2 cells against HR injury and can improve mitochondrial energy metabolism and mitochondrial function integrity. The present study scrutinized the cardioprotective effects of ABLP against HR-induced H9c2 cell injury through antioxidant and mitochondrial pathways. CONCLUSIONS: ABLP could be a promising therapeutic drug for the treatment of myocardial ischemic cardiovascular disease. The results will provide reasonable information for the clinical use of ABLP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión Miocárdica / Miocitos Cardíacos / Medicina Tradicional de Asia Oriental Límite: Animals Idioma: En Revista: Mol Biol Rep Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Daño por Reperfusión Miocárdica / Miocitos Cardíacos / Medicina Tradicional de Asia Oriental Límite: Animals Idioma: En Revista: Mol Biol Rep Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos