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SIRT1 Attenuates Apoptosis of Nucleus Pulposus Cells by Targeting Interactions between LC3B and Fas under High-Magnitude Compression.
Zhuo, Yunyun; Wang, Haoming; Zou, Luetao; Wang, Yiyang; Hu, Yanzhu; Li, Pei; Zhou, Qiang.
Afiliación
  • Zhuo Y; Department of Orthopedics, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
  • Wang H; Department of Orthopedics, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
  • Zou L; Department of Orthopedics, Three Gorges Central Hospital, Chongqing 404000, China.
  • Wang Y; Department of Orthopedics, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
  • Hu Y; Department of Orthopedics, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
  • Li P; Tissue Repairing and Biotechnology Research Center, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China.
  • Zhou Q; Department of Orthopedics, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Oxid Med Cell Longev ; 2021: 2420969, 2021.
Article en En | MEDLINE | ID: mdl-34987698
Mechanical overloading-induced nucleus pulposus cell (NPC) apoptosis plays a core role in the pathogenesis of intervertebral disc degeneration. In this study, we investigated the involvement of mammalian silent information regulator 2 homolog (SIRT1) in NPC apoptosis under high-magnitude compression. Our results showed that high-magnitude compression aggravated cellular apoptosis and attenuated the expression levels of SIRT1 and microtubule-associated protein-1 light chain-3B (LC3B) in rat NPCs in a three-dimensional (3D) cell culture model and an in vivo rat tail compression model, whereas SIRT1 overexpression in NPCs partially reversed these indicators. Moreover, SIRT1 overexpression increased the formation of the LC3B/Fas complex, alleviated activation of the NF-κB pathway, and reduced NPC apoptosis. Finally, downregulation of LC3B partially activated the NF-κB pathway and aggravated NPC apoptosis. Overall, upregulation of SIRT1 increases formation of the LC3B/Fas complex, which contributes to suppression of NPC apoptosis by inhibiting the NF-κB pathway under high compressive stress.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptor fas / Sirtuina 1 / Proteínas Asociadas a Microtúbulos Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: Oxid Med Cell Longev Asunto de la revista: METABOLISMO Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptor fas / Sirtuina 1 / Proteínas Asociadas a Microtúbulos Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: Oxid Med Cell Longev Asunto de la revista: METABOLISMO Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos