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Hsa_circ_0003258 promotes prostate cancer metastasis by complexing with IGF2BP3 and sponging miR-653-5p.
Yu, Yu-Zhong; Lv, Dao-Jun; Wang, Chong; Song, Xian-Lu; Xie, Tao; Wang, Tao; Li, Zhi-Min; Guo, Jia-Ding; Fu, Du-Jiang; Li, Kang-Jin; Wu, Ding-Lan; Chan, Franky Leung; Feng, Ning-Han; Chen, Zhe-Sheng; Zhao, Shan-Chao.
Afiliación
  • Yu YZ; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • Lv DJ; Department of Urology, the Third Affiliated Hospital of Southern Medical University, Guangzhou, 510500, China.
  • Wang C; Department of Urology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.
  • Song XL; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • Xie T; Department of Urology, the Third Affiliated Hospital of Southern Medical University, Guangzhou, 510500, China.
  • Wang T; Department of Radiotherapy, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, 510095, China.
  • Li ZM; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • Guo JD; Department of Urology, the Third Affiliated Hospital of Southern Medical University, Guangzhou, 510500, China.
  • Fu DJ; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • Li KJ; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • Wu DL; Department of Urology, the Third Affiliated Hospital of Southern Medical University, Guangzhou, 510500, China.
  • Chan FL; Department of Urology, the Third Affiliated Hospital of Southern Medical University, Guangzhou, 510500, China.
  • Feng NH; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • Chen ZS; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
  • Zhao SC; Shenzhen Key Laboratory of Viral Oncology, The Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, 518110, China.
Mol Cancer ; 21(1): 12, 2022 01 05.
Article en En | MEDLINE | ID: mdl-34986849
BACKGROUND: More and more studies have shown that circular RNAs (circRNAs) play a critical regulatory role in many cancers. However, the potential molecular mechanism of circRNAs in prostate cancer (PCa) remains largely unknown. METHODS: Differentially expressed circRNAs were identified by RNA sequencing. The expression of hsa_circ_0003258 was evaluated using quantitative real-time PCR and RNA in situ hybridization. The impacts of hsa_circ_0003258 on the metastasis of PCa cells were investigated by a series of in vitro and in vivo assays. Lastly, the underlying mechanism of hsa_circ_0003258 was revealed by Western blot, biotin-labeled RNA pulldown, RNA immunoprecipitation, luciferase assays and rescue experiments. RESULTS: Increased expression of hsa_circ_0003258 was found in PCa tissues and was associated with advanced TNM stage and ISUP grade. Overexpression of hsa_circ_0003258 promoted PCa cell migration by inducing epithelial mesenchymal transformation (EMT) in vitro as well as tumor metastasis in vivo, while knockdown of hsa_circ_0003258 exerts the opposite effect. Mechanistically, hsa_circ_0003258 could elevate the expression of Rho GTPase activating protein 5 (ARHGAP5) via sponging miR-653-5p. In addition, hsa_circ_0003258 physically binds to insulin like growth factor 2 mRNA binding protein 3 (IGF2BP3) in the cytoplasm and enhanced HDAC4 mRNA stability, in which it activates ERK signalling pathway, then triggers EMT programming and finally accelerates the metastasis of PCa. CONCLUSIONS: Upregulation of hsa_circ_0003258 drives tumor progression through both hsa_circ_0003258/miR-653-5p/ARHGAP5 axis and hsa_circ_0003258/IGF2BP3 /HDAC4 axis. Hsa_circ_0003258 may act as a promising biomarker for metastasis of PCa and an attractive target for PCa intervention.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Regulación Neoplásica de la Expresión Génica / Proteínas de Unión al ARN / MicroARNs / Interferencia de ARN / ARN Circular Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Aged / Aged80 / Animals / Humans / Male / Middle aged Idioma: En Revista: Mol Cancer Asunto de la revista: NEOPLASIAS Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Regulación Neoplásica de la Expresión Génica / Proteínas de Unión al ARN / MicroARNs / Interferencia de ARN / ARN Circular Tipo de estudio: Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Aged / Aged80 / Animals / Humans / Male / Middle aged Idioma: En Revista: Mol Cancer Asunto de la revista: NEOPLASIAS Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido