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J-Edited DIffusional Proton Nuclear Magnetic Resonance Spectroscopic Measurement of Glycoprotein and Supramolecular Phospholipid Biomarkers of Inflammation in Human Serum.
Nitschke, Philipp; Lodge, Samantha; Kimhofer, Torben; Masuda, Reika; Bong, Sze-How; Hall, Drew; Schäfer, Hartmut; Spraul, Manfred; Pompe, Nils; Diercks, Tammo; Bernardo-Seisdedos, Ganeko; Mato, José M; Millet, Oscar; Susic, Daniella; Henry, Amanda; El-Omar, Emad M; Holmes, Elaine; Lindon, John C; Nicholson, Jeremy K; Wist, Julien.
Afiliación
  • Nitschke P; Australian National Phenome Centre and Computational and Systems Medicine, Health Futures Institute, Murdoch University, Harry Perkins Building, Perth, Western Australia 6150, Australia.
  • Lodge S; Australian National Phenome Centre and Computational and Systems Medicine, Health Futures Institute, Murdoch University, Harry Perkins Building, Perth, Western Australia 6150, Australia.
  • Kimhofer T; Australian National Phenome Centre and Computational and Systems Medicine, Health Futures Institute, Murdoch University, Harry Perkins Building, Perth, Western Australia 6150, Australia.
  • Masuda R; Australian National Phenome Centre and Computational and Systems Medicine, Health Futures Institute, Murdoch University, Harry Perkins Building, Perth, Western Australia 6150, Australia.
  • Bong SH; Australian National Phenome Centre and Computational and Systems Medicine, Health Futures Institute, Murdoch University, Harry Perkins Building, Perth, Western Australia 6150, Australia.
  • Hall D; Australian National Phenome Centre and Computational and Systems Medicine, Health Futures Institute, Murdoch University, Harry Perkins Building, Perth, Western Australia 6150, Australia.
  • Schäfer H; Bruker Biospin GmbH, Silberstreifen, 76287, Rheinstetten 76287, Germany.
  • Spraul M; Bruker Biospin GmbH, Silberstreifen, 76287, Rheinstetten 76287, Germany.
  • Pompe N; Bruker Biospin GmbH, Silberstreifen, 76287, Rheinstetten 76287, Germany.
  • Diercks T; Precision Medicine and Metabolism Laboratory, CIC bioGUNE, Parque Tecnológico de Bizkaia, Bld. 800, 48160, Derio 48160, Spain.
  • Bernardo-Seisdedos G; Precision Medicine and Metabolism Laboratory, CIC bioGUNE, Parque Tecnológico de Bizkaia, Bld. 800, 48160, Derio 48160, Spain.
  • Mato JM; Precision Medicine and Metabolism Laboratory, CIC bioGUNE, Parque Tecnológico de Bizkaia, Bld. 800, 48160, Derio 48160, Spain.
  • Millet O; Precision Medicine and Metabolism Laboratory, CIC bioGUNE, Parque Tecnológico de Bizkaia, Bld. 800, 48160, Derio 48160, Spain.
  • Susic D; School of Women's and Children's Health, University of New South Wales, Sydney, New South Wales 2052, Australia.
  • Henry A; UNSW Microbiome Research Centre, St George Hospital, Kogarah, New South Wales 2217, Australia.
  • El-Omar EM; School of Women's and Children's Health, University of New South Wales, Sydney, New South Wales 2052, Australia.
  • Holmes E; UNSW Microbiome Research Centre, St George Hospital, Kogarah, New South Wales 2217, Australia.
  • Lindon JC; Microbiome Research Centre, St George & Sutherland Clinical School, University of New South Wales, Sydney, New South Wales 2052, Australia.
  • Nicholson JK; Australian National Phenome Centre and Computational and Systems Medicine, Health Futures Institute, Murdoch University, Harry Perkins Building, Perth, Western Australia 6150, Australia.
  • Wist J; Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington, London SW7 2AZ, U.K.
Anal Chem ; 94(2): 1333-1341, 2022 01 18.
Article en En | MEDLINE | ID: mdl-34985268
Proton nuclear magnetic resonance (NMR) N-acetyl signals (Glyc) from glycoproteins and supramolecular phospholipids composite peak (SPC) from phospholipid quaternary nitrogen methyls in subcompartments of lipoprotein particles) can give important systemic metabolic information, but their absolute quantification is compromised by overlap with interfering resonances from lipoprotein lipids themselves. We present a J-Edited DIffusional (JEDI) proton NMR spectroscopic approach to selectively augment signals from the inflammatory marker peaks Glyc and SPCs in blood serum NMR spectra, which enables direct integration of peaks associated with molecules found in specific compartments. We explore a range of pulse sequences that allow editing based on peak J-modulation, translational diffusion, and T2 relaxation time and validate them for untreated blood serum samples from SARS-CoV-2 infected patients (n = 116) as well as samples from healthy controls and pregnant women with physiological inflammation and hyperlipidemia (n = 631). The data show that JEDI is an improved approach to selectively investigate inflammatory signals in serum and may have widespread diagnostic applicability to disease states associated with systemic inflammation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Protones / COVID-19 Límite: Female / Humans / Pregnancy Idioma: En Revista: Anal Chem Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Protones / COVID-19 Límite: Female / Humans / Pregnancy Idioma: En Revista: Anal Chem Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos