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Ceria nanoparticles ameliorate renal fibrosis by modulating the balance between oxidative phosphorylation and aerobic glycolysis.
Wang, Mengling; Zeng, Feng; Ning, Fengling; Wang, Yinhang; Zhou, Shilin; He, Jiaqi; Li, Cong; Wang, Cong; Sun, Xiaolin; Zhang, Dongliang; Xiao, Jisheng; Hu, Ping; Reilly, Svetlana; Xin, Hong; Xu, Xudong; Zhang, Xuemei.
Afiliación
  • Wang M; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China.
  • Zeng F; Artemisinin Research Center, Institute of Science and Technology, The First Clinical Medical School, Lingnan Medical Research Center, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
  • Ning F; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China.
  • Wang Y; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China.
  • Zhou S; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China.
  • He J; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China.
  • Li C; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China.
  • Wang C; Key Laboratory of Smart Drug Delivery, Ministry of Education, School of Pharmacy, Fudan University, Shanghai, China, Academy for Engineering and Technology, Fudan University, 20 Handan Road, Yangpu District, Shanghai, 200433, China.
  • Sun X; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China.
  • Zhang D; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China.
  • Xiao J; Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
  • Hu P; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China.
  • Reilly S; Division of Cardiovascular Medicine, Department of Medicine, University of Oxford, John Radcliffe Hospital, RadcliffeOxford, UK.
  • Xin H; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China. xinhong@fudan.edu.cn.
  • Xu X; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China. xxdmzx@sina.com.
  • Zhang X; Department of Pharmacology, School of Pharmacy, Minhang Hospital, Fudan University, Shanghai, 201203, China. xuemzhang@fudan.edu.cn.
J Nanobiotechnology ; 20(1): 3, 2022 Jan 04.
Article en En | MEDLINE | ID: mdl-34983531
BACKGROUND AND AIMS: Renal fibrosis is the common outcome in all progressive forms of chronic kidney disease. Unfortunately, the pathogenesis of renal fibrosis remains largely unexplored, among which metabolic reprogramming plays an extremely crucial role in the evolution of renal fibrosis. Ceria nanoparticles (CeNP-PEG) with strong ROS scavenging and anti-inflammatory activities have been applied for mitochondrial oxidative stress and inflammatory diseases. The present study aims to determine whether CeNP-PEG has therapeutic value for renal fibrosis. METHODS: The unilateral ureteral obstructive fibrosis model was used to assess the therapeutic effects in vivo. Transforming growth factor beta1-induced epithelial-to-mesenchymal transition in HK-2 cells was used as the in vitro cell model. The seahorse bioscience X96 extracellular flux analyzer was used to measure the oxygen consumption rate and extracellular acidification rate. RESULTS: In the present study, CeNP-PEG treatment significantly ameliorated renal fibrosis by increased E-cadherin protein expression, and decreased α-SMA, Vimentin and Fibronectin expression both in vitro and in vivo. Additionally, CeNP-PEG significantly reduced the ROS formation and improved the levels of mitochondrial ATP. The seahorse analyzer assay demonstrated that the extracellular acidification rate markedly decreased, whereas the oxygen consumption rate markedly increased, in the presence of CeNP-PEG. Furthermore, the mitochondrial membrane potential markedly enhanced, hexokinase 1 and hexokinase 2 expression significantly decreased after treatment with CeNP-PEG. CONCLUSIONS: CeNP-PEG can block the dysregulated metabolic status and exert protective function on renal fibrosis. This may provide another therapeutic option for renal fibrosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosforilación Oxidativa / Cerio / Nanopartículas del Metal / Glucólisis / Riñón Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: J Nanobiotechnology Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosforilación Oxidativa / Cerio / Nanopartículas del Metal / Glucólisis / Riñón Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: J Nanobiotechnology Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido