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The X-ray crystal structure of human A15C neuroglobin reveals both native/de novo disulfide bonds and unexpected ligand-binding sites.
Gao, Shu-Qin; Yuan, Hong; Liu, Xi-Chun; Li, Lianzhi; Tan, Xiangshi; Wen, Ge-Bo; Lin, Ying-Wu.
Afiliación
  • Gao SQ; Hengyang Medical School, University of South China, Hengyang, China.
  • Yuan H; Department of Chemistry & Institute of Biomedical Science, Fudan University, Shanghai, China.
  • Liu XC; School of Chemistry and Chemical Engineering, University of South China, Hengyang, China.
  • Li L; School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, China.
  • Tan X; Department of Chemistry & Institute of Biomedical Science, Fudan University, Shanghai, China.
  • Wen GB; Hengyang Medical School, University of South China, Hengyang, China.
  • Lin YW; Hengyang Medical School, University of South China, Hengyang, China.
Proteins ; 90(5): 1152-1158, 2022 05.
Article en En | MEDLINE | ID: mdl-34982478
Human neuroglobin (Ngb) contains a heme group and three Cys residues (Cys46, Cys55, and Cys120) in the polypeptide chain. By introducing an additional Cys at position 15, the X-ray structure of A15C Ngb mutant was solved at a high resolution of 1.35 Å, which reveals the formation of both the native (C46C55) and the engineered (C15C120) disulfide bonds, likely playing a functional and structural role, respectively, according to the geometry analysis. Unexpectedly, 1,4-dioxane from the crystallization reagents was bound not only to the protein surface, but also to the heme distal pocket, providing insights into protein-ligand interactions for the globin and guiding the design of functional heme enzymes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Globinas / Proteínas del Tejido Nervioso Límite: Humans Idioma: En Revista: Proteins Asunto de la revista: BIOQUIMICA Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Globinas / Proteínas del Tejido Nervioso Límite: Humans Idioma: En Revista: Proteins Asunto de la revista: BIOQUIMICA Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos