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CryoEM analysis of small plant biocatalysts at sub-2 Šresolution.
Dimos, Nicole; Helmer, Carl P O; Chánique, Andrea M; Wahl, Markus C; Kourist, Robert; Hilal, Tarek; Loll, Bernhard.
Afiliación
  • Dimos N; Institute of Chemistry and Biochemistry, Department of Biology, Chemistry, Pharmacy, Laboratory of Structural Biochemistry, Free University of Berlin, Takustrasse 6, 14195 Berlin, Germany.
  • Helmer CPO; Institute of Chemistry and Biochemistry, Department of Biology, Chemistry, Pharmacy, Laboratory of Structural Biochemistry, Free University of Berlin, Takustrasse 6, 14195 Berlin, Germany.
  • Chánique AM; Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, 8010 Graz, Austria.
  • Wahl MC; Institute of Chemistry and Biochemistry, Department of Biology, Chemistry, Pharmacy, Laboratory of Structural Biochemistry, Free University of Berlin, Takustrasse 6, 14195 Berlin, Germany.
  • Kourist R; Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse 14, 8010 Graz, Austria.
  • Hilal T; Institute of Chemistry and Biochemistry, Research Center of Electron Microscopy and Core Facility BioSupraMol, Free University of Berlin, Fabeckstrasse 36A, 14195 Berlin, Germany.
  • Loll B; Institute of Chemistry and Biochemistry, Department of Biology, Chemistry, Pharmacy, Laboratory of Structural Biochemistry, Free University of Berlin, Takustrasse 6, 14195 Berlin, Germany.
Acta Crystallogr D Struct Biol ; 78(Pt 1): 113-123, 2022 Jan 01.
Article en En | MEDLINE | ID: mdl-34981767
Enzyme catalysis has emerged as a key technology for developing efficient, sustainable processes in the chemical, biotechnological and pharmaceutical industries. Plants provide large and diverse pools of biosynthetic enzymes that facilitate complex reactions, such as the formation of intricate terpene carbon skeletons, with exquisite specificity. High-resolution structural analysis of these enzymes is crucial in order to understand their mechanisms and modulate their properties by targeted engineering. Although cryo-electron microscopy (cryoEM) has revolutionized structural biology, its applicability to high-resolution structural analysis of comparatively small enzymes has so far been largely unexplored. Here, it is shown that cryoEM can reveal the structures of plant borneol dehydrogenases of ∼120 kDa at or below 2 Šresolution, paving the way for the rapid development of new biocatalysts that can provide access to bioactive terpenes and terpenoids.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plantas / Catálisis / Microscopía por Crioelectrón / Enzimas Idioma: En Revista: Acta Crystallogr D Struct Biol Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plantas / Catálisis / Microscopía por Crioelectrón / Enzimas Idioma: En Revista: Acta Crystallogr D Struct Biol Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos