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Preparation and evaluation of polycaprolactone/chitosan/Jaft biocompatible nanofibers as a burn wound dressing.
Hashemi, Seyedeh-Sara; Saadatjo, Zohreh; Mahmoudi, Reza; Delaviz, Hamdollah; Bardania, Hassan; Rajabi, Seyedeh-Somayeh; Rafati, Alireza; Zarshenas, Mohammad M; Barmak, Mehrzad Jafari.
Afiliación
  • Hashemi SS; Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Saadatjo Z; Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
  • Mahmoudi R; Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran. Electronic address: rmahmoudi40@yahoo.com.
  • Delaviz H; Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran. Electronic address: delavizhamdi83@gmail.com.
  • Bardania H; Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran. Electronic address: hbardania@gmail.com.
  • Rajabi SS; Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
  • Rafati A; Division of Pharmacology and Pharmaceutical Chemistry, Sarvestan Branch, Islamic Azad University, Sarvestan, Iran.
  • Zarshenas MM; Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; Epilepsy Research Center, Shiraz University of Medical Sciences, Shira
  • Barmak MJ; Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran. Electronic address: mehrzadj14@gmail.com.
Burns ; 48(7): 1690-1705, 2022 11.
Article en En | MEDLINE | ID: mdl-34973854
Tissue engineering is an emerging method for replacing damaged tissues. In this study, the potential application of electrospun polycaprolactone/chitosan/ the internal layer of oak fruit (Jaft) as skin scaffolds was investigated. A combination of Polycaprolactone (PCL), chitosan (CH), and the internal layer of oak fruit (Jaft) was used to incorporate mechanical properties of synthetic polymers, biological properties of natural polymers, and antibacterial activity of Jaft. Physical and morphological characteristics of prepared scaffolds were investigated using a scanning electron microscope (SEM), mechanical analysis, swelling ratio, and contact angle. Moreover, chemical and biological properties were evaluated by Fourier-transform infrared spectroscopy (FTIR), chromatography, flow cytometry, DAPI staining, MTT assay, and trypan blue exclusion assay. Obtained results demonstrated that the fabricated scaffolds have good mechanical properties. Moreover, the addition of chitosan and Jaft to the PCL scaffolds improved their water absorption capacity as well as surface hydrophilicity. MTT results showed the fabricated nanofibrous scaffolds have adequate cell viability, which is higher than the cell culture plate at each time point of culture. Furthermore, SEM images of cultured scaffolds, trypan blue exclusion assay, and DAPI staining confirmed that fibroblast cells could be well-attached and proliferate on the PCL/CH/Jaft scaffolds. Results have proven that this novel bioactive scaffold has promising mechanical properties, suitable biocompatibility in vitro, and in vivo. Consequently, it could be a promising candidate for skin tissue engineering applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quemaduras / Quitosano / Nanofibras Límite: Humans Idioma: En Revista: Burns Asunto de la revista: TRAUMATOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quemaduras / Quitosano / Nanofibras Límite: Humans Idioma: En Revista: Burns Asunto de la revista: TRAUMATOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos