Preparation and structural properties of selenium modified heteropolysaccharide from the fruits of Akebia quinata and in vitro and in vivo antitumor activity.

Wang, Huimei; Li, Ying; Wang, Xuelian; Li, Yuhao; Cui, Jianlin; Jin, Da-Qing; Tuerhong, Muhetaer; Abudukeremu, Munira; Xu, Jing; Guo, Yuanqiang

Wang, Huimei; Li, Ying; Wang, Xuelian; Li, Yuhao; Cui, Jianlin; Jin, Da-Qing; Tuerhong, Muhetaer; Abudukeremu, Munira; Xu, Jing; Guo, Yuanqiang.

Afiliación

Wang H; State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
Li Y; State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
Wang X; State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
Li Y; School of Medicine, Nankai University, Tianjin 300071, People's Republic of China.
Cui J; School of Medicine, Nankai University, Tianjin 300071, People's Republic of China.
Jin DQ; School of Medicine, Nankai University, Tianjin 300071, People's Republic of China.
Tuerhong M; College of Chemistry and Environmental Sciences, Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry, Kashgar University, Kashgar 844000, People's Republic of China.
Abudukeremu M; College of Chemistry and Environmental Sciences, Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry, Kashgar University, Kashgar 844000, People's Republic of China.
Xu J; State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China; State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, G
Guo Y; State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China. Electronic address: victgyq@nankai.edu.cn.

Carbohydr Polym ; 278: 118950, 2022 Feb 15.

Article en En | MEDLINE | ID: mdl-34973766

RESUMEN

Cancer is a complex disease, and blocking tumor angiogenesis has become one of the most promising approaches in cancer therapy. Here, an exopoly heteropolysaccharide (AQP70-2B) was firstly isolated from Akebia quinata. Monosaccharide composition indicated that the AQP70-2B was composed of rhamnose, glucose, galactose, and arabinose. The backbone of AQP70-2B consisted of â1)-l-Araf, â3)-l-Araf-(1â, â5)-l-Araf-(1â, â3,5)-l-Araf-(1â, â2,5)-l-Araf-(1â, â4)-d-Glcp-(1â, â6)-d-Galp-(1â, and â1)-d-Rhap residues. Based on the close relationship between selenium and anti-tumor activity, AQP70-2B was modified with selenium to obtain selenized polysaccharide Se-AQP70-2B. Then, a series of methods for analysis and characterization, especially scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS), indicated that Se-AQP70-2B was successfully synthesized. Furthermore, zebrafish xenografts and anti-angiogenesis experiments indicated that selenization could improve the antitumor activity by inhibiting tumor cell proliferation and migration and blocking angiogenesis.

Asunto(s)

Inhibidores de la Angiogénesis/farmacología; Antineoplásicos Fitogénicos/farmacología; Frutas/química; Neovascularización Patológica/tratamiento farmacológico; Polisacáridos/farmacología; Ranunculales/química; Selenio/química; Inhibidores de la Angiogénesis/química; Inhibidores de la Angiogénesis/aislamiento & purificación; Animales; Antineoplásicos Fitogénicos/química; Antineoplásicos Fitogénicos/aislamiento & purificación; Conformación de Carbohidratos; Movimiento Celular/efectos de los fármacos; Proliferación Celular/efectos de los fármacos; Ensayos de Selección de Medicamentos Antitumorales; Células Hep G2; Humanos; Neoplasias Hepáticas Experimentales/tratamiento farmacológico; Neoplasias Hepáticas Experimentales/patología; Neovascularización Patológica/patología; Polisacáridos/química; Polisacáridos/aislamiento & purificación; Pez Cebra/embriología

Palabras clave

Akebia quinata; Antitumor activity; Dichloromethane (PubChem CID: 6344); Ethanol (PubChem CID: 702); Heteropolysaccharide; Methyl iodide (PubChem CID: 6328); Neutral red (PubChem CID: 11105); O-tolylisothiocyanate (PubChem CID: 341280687); Selenylation; Sodium chloride (PubChem CID: 5234); Sodium dihydrogen phosphate (PubChem CID: 23672064); Structure characterization; d-Glucose (PubChem CID: 5793); d-Mannose (PubChem CID: 18950); l-Arabinose (PubChem CID: 439195)

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Selenio / Inhibidores de la Angiogénesis / Ranunculales / Frutas / Neovascularización Patológica / Antineoplásicos Fitogénicos Límite: Animals / Humans Idioma: En Revista: Carbohydr Polym Año: 2022 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google