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Peri-implantation expression and regulation of ITGB8 in goat uterus.
Bu, Li-Ge; Sun, Ya; Li, Ting-Yue; Kong, Li-Li; Yu, Hao-Nan; Li, Shi-Jie; Ding, Nai-Zheng; Ni, Hua.
Afiliación
  • Bu LG; The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, 150030, China.
  • Sun Y; The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, 150030, China.
  • Li TY; The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, 150030, China.
  • Kong LL; The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, 150030, China.
  • Yu HN; The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, 150030, China.
  • Li SJ; The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, 150030, China.
  • Ding NZ; Key Laboratory of Animal Resistance Biology of Shandong Province, College of Life Science, Shandong Normal University, Jinan, 250014, China.
  • Ni H; The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin, 150030, China. Electronic address: huani@neau.edu.cn.
Theriogenology ; 180: 130-136, 2022 Mar 01.
Article en En | MEDLINE | ID: mdl-34973644
Ruminants have a superficial implantation pattern. The extended conceptus attaches to the receptive endometrium to form the cotyledonary placenta. During the attachment, a large number of events occur at the maternal-fetal interface. However, the related molecular mechanisms have not been fully understood. Integrin beta8 (ITGB8) is a subunit of integrin beta involved in embryo implantation. In this study, we determined peri-implantation expression and regulation of ITGB8 in goat uterus. The mRNA and protein levels of ITGB8 were both high in goat endometrial luminal epithelium (LE) and superficial glandular epithelium (sGE) during the adhesion period (Days 16-19 of pregnancy). Such expression profile was opposite to that of microRNA-187 (miR-187). Then, we validated that miR-187 targeted the 3' untranslated region (UTR) of ITGB8 in primary goat endometrial epithelial cells (EECs). In EECs, inhibition of miR-187 resulted in not only up-regulated ITGB8 level but also reduced cell proliferation and focal adhesion kinase (FAK) activity. Moreover, ITGB8 and miR-187 were regulated by interferon tau (IFNT). Altogether, in goat, the miR-187/ITGB8 axis may be involved in conceptus attachment and is downstream of IFNT. Our results will help us better understand the mechanisms of ruminant implantation and may provide a useful tool to improve the reproduction ratio for ruminants.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Útero / Cabras / Interferón Tipo I / Cadenas beta de Integrinas Límite: Animals / Pregnancy Idioma: En Revista: Theriogenology Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Útero / Cabras / Interferón Tipo I / Cadenas beta de Integrinas Límite: Animals / Pregnancy Idioma: En Revista: Theriogenology Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos