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Design of a naphthalimide-based probe for acrolein detection in foods and cells.
Jiang, Kaiyu; Zhou, Ping; Zheng, Jie; Huang, Caihuan; Hu, Jiaman; Guo, Hongyang; Ou, Juanying; Ou, Shiyi.
Afiliación
  • Jiang K; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
  • Zhou P; InnoStar Bio-tech Nantong Co., Ltd., Nantong 226133, China.
  • Zheng J; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
  • Huang C; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
  • Hu J; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
  • Guo H; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
  • Ou J; Institute of Food Safety & Nutrition, Jinan University, Guangzhou 510632, China; Guangdong-Hong Kong Joint Innovation Platform for the Safety of Bakery Products, Guangzhou 510632, China. Electronic address: ojy_26@163.com.
  • Ou S; Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Guangdong-Hong Kong Joint Innovation Platform for the Safety of Bakery Products, Guangzhou 510632, China. Electronic address: tosy@jnu.edu.cn.
J Hazard Mater ; 426: 128118, 2022 03 15.
Article en En | MEDLINE | ID: mdl-34968849
Acrolein is a highly toxic agent that can be generated exogenously and endogenously. Therefore, a highly specific and sensitive probe for acrolein with potential applications in acrolein detection must be developed. In this research, a novel fluorescent probe named "probe for acrolein detection" (Pr-ACR) was designed and synthesized based on a naphthalimide fluorophore skeleton, and a thiol group (-SH) was introduced into its structure for acrolein recognition. The -SH traps acrolein via Michael addition and the resultant interaction product of the probe inhibits the photoinduced electron transfer process and produce a strong fluorescence at 510 nm. The probe showed high sensitivity and specificity for acrolein. HPLC-MS/MS analysis verified that it can be used to quantify acrolein in foods, such as soda crackers, red wine, and baijiu, with a fluorescence spectrophotometer. After methyl esterification, the methyl esterified probe (mPr-ACR) successfully visualised acrolein in Hela cells under a laser scanning confocal microscope. This finding proved that Pr-ACR and mPr-ACR are potential tools for the detection and visualisation of acrolein from different sources.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acroleína / Naftalimidas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Hazard Mater Asunto de la revista: SAUDE AMBIENTAL Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acroleína / Naftalimidas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Hazard Mater Asunto de la revista: SAUDE AMBIENTAL Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos