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An Outbreak in Pigeons Caused by the Subgenotype VI.2.1.2 of Newcastle Disease Virus in Brazil.
Thomazelli, Luciano M; Sinhorini, Juliana A; Oliveira, Danielle B L; Knöbl, Terezinha; Bosqueiro, Tatiana C M; Sano, Elder; Costa, Gladyston C V; Monteiro, Cairo; Dorlass, Erick G; Utecht, Nathalia; Scagion, Guilherme P; Meneguin, Carla; Silva, Laura M N; Moraes, Maria Vitória S; Bueno, Larissa M; Reischak, Dilmara; Carrasco, Adriano O T; Arns, Clarice W; Ferreira, Helena L; Durigon, Edison L.
Afiliación
  • Thomazelli LM; Biomedical Science Institute, University of São Paulo, São Paulo 05508-000, Brazil.
  • Sinhorini JA; Zoonoses Surveillance Division of the Health Surveillance Coordination, Health Department of São Paulo, R. Santa Eulália, 86, São Paulo 02031-020, Brazil.
  • Oliveira DBL; Biomedical Science Institute, University of São Paulo, São Paulo 05508-000, Brazil.
  • Knöbl T; Hospital Israelita Albert Einstein, São Paulo 05652-900, Brazil.
  • Bosqueiro TCM; Avian Medicine Laboratory, Veterinary Medicine and Animal Science School, University of São Paulo, Butantã, São Paulo 05508-270, Brazil.
  • Sano E; Zoonoses Surveillance Division of the Health Surveillance Coordination, Health Department of São Paulo, R. Santa Eulália, 86, São Paulo 02031-020, Brazil.
  • Costa GCV; Biomedical Science Institute, University of São Paulo, São Paulo 05508-000, Brazil.
  • Monteiro C; Zoonoses Surveillance Division of the Health Surveillance Coordination, Health Department of São Paulo, R. Santa Eulália, 86, São Paulo 02031-020, Brazil.
  • Dorlass EG; Zoonoses Surveillance Division of the Health Surveillance Coordination, Health Department of São Paulo, R. Santa Eulália, 86, São Paulo 02031-020, Brazil.
  • Utecht N; Biomedical Science Institute, University of São Paulo, São Paulo 05508-000, Brazil.
  • Scagion GP; Biomedical Science Institute, University of São Paulo, São Paulo 05508-000, Brazil.
  • Meneguin C; Biomedical Science Institute, University of São Paulo, São Paulo 05508-000, Brazil.
  • Silva LMN; Biomedical Science Institute, University of São Paulo, São Paulo 05508-000, Brazil.
  • Moraes MVS; Biomedical Science Institute, University of São Paulo, São Paulo 05508-000, Brazil.
  • Bueno LM; Graduate Program in Experimental Epidemiology Applied to Zoonoses, Veterinary Medicine and Animal Science School, University of São Paulo, São Paulo 05508-270, Brazil.
  • Reischak D; Graduate Program in Experimental Epidemiology Applied to Zoonoses, Veterinary Medicine and Animal Science School, University of São Paulo, São Paulo 05508-270, Brazil.
  • Carrasco AOT; Department of Veterinary Medicine, FZEA-USP, University of Sao Paulo, Pirassununga 13635-900, Brazil.
  • Arns CW; Federal Laboratory for Agricultural Defense in Sao Paulo (LFDA-SP), Animal Diagnostics Unit, Rua Raul Ferrari, s/n°, Campinas 13100-105, Brazil.
  • Ferreira HL; Department of Veterinary Medicine, State University of the Midwest, Alameda Élio Antonio Dalla Vecchia, 838, Guarapuava 85040-167, Brazil.
  • Durigon EL; Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas-UNICAMP, P.O. Box 6109, Campinas 13083-970, Brazil.
Viruses ; 13(12)2021 12 06.
Article en En | MEDLINE | ID: mdl-34960715
Newcastle disease virus (NDV) can infect over 250 bird species with variable pathogenicity; it can also infect humans in rare cases. The present study investigated an outbreak in feral pigeons in São Paulo city, Brazil, in 2019. Affected birds displayed neurological signs, and hemorrhages were observed in different tissues. Histopathology changes with infiltration of mononuclear inflammatory cells were also found in the brain, kidney, proventriculus, heart, and spleen. NDV staining was detected by immunohistochemistry. Twenty-seven out of thirty-four tested samples (swabs and tissues) were positive for Newcastle disease virus by RT-qPCR test, targeting the M gene. One isolate, obtained from a pool of positive swab samples, was characterized by the intracerebral pathogenicity index (ICPI) and the hemagglutination inhibition (HI) tests. This isolate had an ICPI of 0.99, confirming a virulent NDV strain. The monoclonal antibody 617/161, which recognizes a distinct epitope in pigeon NDV strains, inhibited the isolate with an HI titer of 512. A complete genome of NDV was obtained using next-generation sequencing. Phylogenetic analysis based on the complete CDS F gene grouped the detected isolate with other viruses from subgenotype VI.2.1.2, class II, including one previously reported in Southern Brazil in 2014. This study reports a comprehensive characterization of the subgenotype VI.2.1.2, which seems to have been circulating in Brazilian urban areas since 2014. Due to the zoonotic risk of NDV, virus surveillance in feral pigeons should also be systematically performed in urban areas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Columbidae / Virus de la Enfermedad de Newcastle / Brotes de Enfermedades / Enfermedad de Newcastle Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Viruses Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Columbidae / Virus de la Enfermedad de Newcastle / Brotes de Enfermedades / Enfermedad de Newcastle Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Viruses Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza