Dipolar Order Parameters in Large Systems With Fast Spinning.

Franks, W Trent; Tatman, Ben P; Trenouth, Jonah; Lewandowski, Józef R

Franks, W Trent; Tatman, Ben P; Trenouth, Jonah; Lewandowski, Józef R.

Afiliación

Franks WT; Department of Physics, University of Warwick, Coventry, United Kingdom.
Tatman BP; Department of Chemistry, University of Warwick, Coventry, United Kingdom.
Trenouth J; Department of Physics, University of Warwick, Coventry, United Kingdom.
Lewandowski JR; Department of Chemistry, University of Warwick, Coventry, United Kingdom.

Front Mol Biosci ; 8: 791026, 2021.

Article en En | MEDLINE | ID: mdl-34957221

RESUMEN

Order parameters are a useful tool for quantifying amplitudes of molecular motions. Here we measure dipolar order parameters by recoupling heteronuclear dipole-dipole couplings under fast spinning. We apply symmetry based recoupling methods to samples spinning under magic angle at 60 kHz by employing a variable flip angle compound inversion pulse. We validate the methods by measuring site-specific 15N-1H order parameters of a microcrystalline protein over a small temperature range and the same protein in a large, precipitated complex with antibody. The measurements of the order parameters in the complex are consistent with the observed protein undergoing overall motion within the assembly.

Palabras clave

dynamics; fast MAS NMR; order parameter; proton detection; recoupling; symmetry

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Mol Biosci Año: 2021 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Suiza

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