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Spatially Resolved Expression of Transposable Elements in Disease and Somatic Tissue with SpatialTE.
Valdebenito-Maturana, Braulio; Guatimosim, Cristina; Carrasco, Mónica Alejandra; Tapia, Juan Carlos.
Afiliación
  • Valdebenito-Maturana B; Núcleo Científico Multidisciplinario, School of Medicine, Universidad de Talca, Campus Talca, Talca 3460000, Chile.
  • Guatimosim C; Departamento de Morfologia, ICB, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil.
  • Carrasco MA; School of Medicine, Universidad de Talca, Campus Talca, Talca 3460000, Chile.
  • Tapia JC; School of Medicine, Universidad de Talca, Campus Talca, Talca 3460000, Chile.
Int J Mol Sci ; 22(24)2021 Dec 20.
Article en En | MEDLINE | ID: mdl-34948421
Spatial transcriptomics (ST) is transforming the way we can study gene expression and its regulation through position-specific resolution within tissues. However, as in bulk RNA-Seq, transposable elements (TEs) are not being studied due to their highly repetitive nature. In recent years, TEs have been recognized as important regulators of gene expression, and thus, TE expression analysis in a spatially resolved manner could further help to understand their role in gene regulation within tissues. We present SpatialTE, a tool to analyze TE expression from ST datasets and show its application in somatic and diseased tissues. The results indicate that TEs have spatially regulated expression patterns and that their expression profiles are spatially altered in ALS disease, indicating that TEs might perform differential regulatory functions within tissue organs. We have made SpatialTE publicly available as open-source software under an MIT license.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Programas Informáticos / Elementos Transponibles de ADN / Biología Computacional / Transcriptoma / Esclerosis Amiotrófica Lateral Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Programas Informáticos / Elementos Transponibles de ADN / Biología Computacional / Transcriptoma / Esclerosis Amiotrófica Lateral Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza