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A Hypercaloric Diet Induces Early Podocyte Damage in Aged, Non-Diabetic Rats.
Seikrit, Claudia; Lausberg, Eva; Buhl, Eva Miriam; Gaspar, Robert; Tabi, Tamas; Heffer, Marija; Ducza, Eszter; Sztojkov-Ivanov, Anita; Seres, Adrienn B; Szucs, Kalman; Ivic, Vedrana; Floege, Jürgen; Vari, Sandor G; Boor, Peter; Klinkhammer, Barbara Mara.
Afiliación
  • Seikrit C; Division of Nephrology and Clinical Immunology, RWTH Aachen University, Aachen, Germany.
  • Lausberg E; Institute of Pathology, University Hospital Aachen, RWTH Aachen University, Aachen, Germany.
  • Buhl EM; Institute of Pathology, University Hospital Aachen, RWTH Aachen University, Aachen, Germany.
  • Gaspar R; Institute of Human Genetics, RWTH Aachen University, Aachen, Germany.
  • Tabi T; Institute of Pathology, University Hospital Aachen, RWTH Aachen University, Aachen, Germany.
  • Heffer M; Electron Microscopy Facility, RWTH Aachen University Hospital, Aachen, Germany.
  • Ducza E; Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, Interdisciplinary Excellence Centre, University of Szeged, Szeged, Hungary.
  • Sztojkov-Ivanov A; Department of Pharmacodynamics, Faculty of Pharmacy, Semmelweis University, Budapest, Hungary.
  • Seres AB; Department of Medical Biology and Genetics, Faculty of Medicine, J. J. Strossmayer University of Osijek, Osijek, Croatia.
  • Szucs K; Department of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, Hungary.
  • Ivic V; Department of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, Hungary.
  • Floege J; Department of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, Hungary.
  • Vari SG; Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, Interdisciplinary Excellence Centre, University of Szeged, Szeged, Hungary.
  • Boor P; Department of Medical Biology and Genetics, Faculty of Medicine, J. J. Strossmayer University of Osijek, Osijek, Croatia.
  • Klinkhammer BM; Division of Nephrology and Clinical Immunology, RWTH Aachen University, Aachen, Germany.
Cell Physiol Biochem ; 55(S4): 96-112, 2021 Dec 23.
Article en En | MEDLINE | ID: mdl-34936286
BACKGROUND/AIMS: The number of patients of older age with metabolic syndrome, obesity, and associated kidney disease, which is characterized by podocyte damage, glomerular hypertrophy, and focal segmental glomerulosclerosis (FSGS), is increasing worldwide. Animal models that would reflect the development of such kidney diseases could facilitate the testing of drugs. We investigated the renal effects of a long-term high caloric diet in aged rats and the potential effects of drugs used to treat metabolic syndrome. METHODS: We analyzed nine-month-old male and female Sprague Dawley rats fed five months with a normal diet (control group) or high-fat-high-carbohydrate diet (HFHCD group). Two additional groups were fed with HFHCD and treated with drugs used in patients with metabolic syndrome, i.e., the glucagon-like peptide receptor 1 agonist liraglutide (HFHCD+liraglutide group) or metformin (HFHCD+metformin group). RESULTS: Except an increase of waist circumference as a sign of visceral obesity, the HFHCD diet did not induce metabolic syndrome or obesity. There were no significant changes in kidney function and all groups showed similar indices of glomerular injury, i.e., no differences in glomerular size or the number of glomeruli with FSGS or with FSGS-precursor lesions quantified by CD44 expression as a marker of parietal epithelial cell (PEC) activation. Analysis of ultrastructural morphology revealed mild podocyte stress and a decrease of glomerular nestin expression in the HFHCD group, whereas podocin and desmin were not altered. HFHCD did not promote fibrogenesis, however, treatment with liraglutide led to a slightly increased tubulointerstitial damage, immune cell infiltration, and collagen IV expression compared to the control and HFHCD groups. CONCLUSION: A five-month feeding with HFHCD in aged rats induced mild podocyte injury and microinflammation, which was not alleviated by liraglutide or metformin.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Síndrome Metabólico / Podocitos / Dieta Alta en Grasa / Enfermedades Renales / Obesidad Límite: Animals Idioma: En Revista: Cell Physiol Biochem Asunto de la revista: BIOQUIMICA / FARMACOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Síndrome Metabólico / Podocitos / Dieta Alta en Grasa / Enfermedades Renales / Obesidad Límite: Animals Idioma: En Revista: Cell Physiol Biochem Asunto de la revista: BIOQUIMICA / FARMACOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania