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Texturing Hierarchical Tissues by Gradient Assembling of Microengineered Platelet-Lysates Activated Fibers.
Calejo, Isabel; Reis, Rui L; Domingues, Rui M A; Gomes, Manuela E.
Afiliación
  • Calejo I; 3B's Research Group, i3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Barco, Guimarães, 4805-017, Portugal.
  • Reis RL; 3B's Research Group, i3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Barco, Guimarães, 4805-017, Portugal.
  • Domingues RMA; 3B's Research Group, i3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Barco, Guimarães, 4805-017, Portugal.
  • Gomes ME; 3B's Research Group, i3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Barco, Guimarães, 4805-017, Portugal.
Adv Healthc Mater ; 11(8): e2102076, 2022 04.
Article en En | MEDLINE | ID: mdl-34927396
The heterogeneity of hierarchical tissues requires designing multipart engineered constructs as suitable tissue replacements. Herein, the incorporation of platelet lysate (PL) within an electrospun fiber core is proposed aiming for the fabrication of functionally graded 3D scaffolds for heterotypic tissues regeneration, such as tendon-to-bone interfaces. First, anisotropic yarns (A-Yarns) and isotropic threads with nanohydroxyapatite (I-Threads/PL@nHAp) are fabricated to recreate the tendon- and bone-microstructures and both incorporated with PL using emulsion electrospinning for a sustained and local delivery of growth factors, cytokines, and chemokines. Biological performance using human adipose-derived stem cells demonstrates that A-Yarns/PL induce a higher expression of scleraxis, a tenogenic-marker, while in I-Threads/PL@nHAp, higher alkaline phosphatase activity and matrix mineralization suggest an osteogenic commitment without the need for biochemical supplementation compared to controls. As a proof-of-concept, functional 3D gradient scaffolds are fabricated using a weaving technique, resulting in 3D textured hierarchical constructs with gradients in composition and topography. Additionally, the precise delivery of bioactive cues together with in situ biophysical features guide the commitment into a phenotypic gradient exhibiting chondrogenic and osteochondrogenic profiles in the interface of scaffolds. Overall, a promising patch solution for the regeneration of tendon-to-bone tissue interface through the fabrication of PL-functional 3D gradient constructs is demonstrated.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Andamios del Tejido Límite: Humans Idioma: En Revista: Adv Healthc Mater Año: 2022 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Andamios del Tejido Límite: Humans Idioma: En Revista: Adv Healthc Mater Año: 2022 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Alemania