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Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication.
Savelyev, Alexander G; Sochilina, Anastasia V; Akasov, Roman A; Mironov, Anton V; Kapitannikova, Alina Yu; Borodina, Tatiana N; Sholina, Natalya V; Khaydukov, Kirill V; Zvyagin, Andrei V; Generalova, Alla N; Khaydukov, Evgeny V.
Afiliación
  • Savelyev AG; Federal Scientific Research Centre "Crystallography and Photonics" Russian Academy of Sciences, Moscow, Russia.
  • Sochilina AV; Center of Biomedical Engineering, Institute of Molecular Medicine, Sechenov University, Moscow, Russia.
  • Akasov RA; Federal Scientific Research Centre "Crystallography and Photonics" Russian Academy of Sciences, Moscow, Russia.
  • Mironov AV; Shemyakin-Ovchinnikov Institute of Bioorganic Сhemistry RAS, Moscow, Russia.
  • Kapitannikova AY; Federal Scientific Research Centre "Crystallography and Photonics" Russian Academy of Sciences, Moscow, Russia.
  • Borodina TN; Center of Biomedical Engineering, Institute of Molecular Medicine, Sechenov University, Moscow, Russia.
  • Sholina NV; Shemyakin-Ovchinnikov Institute of Bioorganic Сhemistry RAS, Moscow, Russia.
  • Khaydukov KV; Federal Scientific Research Centre "Crystallography and Photonics" Russian Academy of Sciences, Moscow, Russia.
  • Zvyagin AV; Center of Biomedical Engineering, Institute of Molecular Medicine, Sechenov University, Moscow, Russia.
  • Generalova AN; Federal Scientific Research Centre "Crystallography and Photonics" Russian Academy of Sciences, Moscow, Russia.
  • Khaydukov EV; Federal Scientific Research Centre "Crystallography and Photonics" Russian Academy of Sciences, Moscow, Russia.
Front Bioeng Biotechnol ; 9: 783834, 2021.
Article en En | MEDLINE | ID: mdl-34926429
Bioprinting emerges as a powerful flexible approach for tissue engineering with prospective capability to produce tissue on demand, including biomimetic hollow-core fiber structures. In spite of significance for tissue engineering, hollow-core structures proved difficult to fabricate, with the existing methods limited to multistage, time-consuming, and cumbersome procedures. Here, we report a versatile cell-friendly photopolymerization approach that enables single-step prototyping of hollow-core as well as solid-core hydrogel fibers initially loaded with living cells. This approach was implemented by extruding cell-laden hyaluronic acid glycidyl methacrylate hydrogel directly into aqueous solution containing free radicals generated by continuous blue light photoexcitation of the flavin mononucleotide/triethanolamine photoinitiator. Diffusion of free radicals from the solution to the extruded structure initiated cross-linking of the hydrogel, progressing from the structure surface inwards. Thus, the cross-linked wall is formed and its thickness is limited by penetration of free radicals in the hydrogel volume. After developing in water, the hollow-core fiber is formed with centimeter range of lengths. Amazingly, HaCaT cells embedded in the hydrogel successfully go through the fabrication procedure. The broad size ranges have been demonstrated: from solid core to 6% wall thickness of the outer diameter, which was variable from sub-millimeter to 6 mm, and Young's modulus ∼1.6 ± 0.4 MPa. This new proof-of-concept fibers photofabrication approach opens lucrative opportunities for facile three-dimensional fabrication of hollow-core biostructures with controllable geometry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Bioeng Biotechnol Año: 2021 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Bioeng Biotechnol Año: 2021 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza