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Evaluation of PD-1 and PD-L1 expression in canine urothelial carcinoma cell lines.
Pinard, Christopher J; Hocker, Samuel E; Poon, Andrew C; Inkol, Jordon M; Matsuyama, Arata; Wood, R Darren; Wood, Geoffrey A; Woods, J Paul; Mutsaers, Anthony J.
Afiliación
  • Pinard CJ; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada. Electronic address: pinardc@uoguelph.ca.
  • Hocker SE; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada; Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS, 66502, USA.
  • Poon AC; Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
  • Inkol JM; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
  • Matsuyama A; Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
  • Wood RD; Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
  • Wood GA; Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
  • Woods JP; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
  • Mutsaers AJ; Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada; Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Vet Immunol Immunopathol ; 243: 110367, 2022 Jan.
Article en En | MEDLINE | ID: mdl-34923192
Urothelial carcinoma (UC) is the most common urinary tumor in dogs and despite combinational therapies, only modest improvements in survival have been achieved in recent years. Given the utility of monoclonal antibodies against PD-1 and PD-L1 in human UC, we evaluated the protein and mRNA expression in three established canine urothelial carcinoma cell lines. Flow cytometry and western blot analysis confirmed cell line expression of both molecules in varying degrees. Reverse transcription PCR (RT-PCR) documented mRNA expression in all three cell lines for both PD-1 and PD-L1. Fluorescence microscopy was consistent with strong PD-1 and PD-L1 expression in the canine cell lines and was in line with previous human literature. Importantly, the flow cytometry work described in this study revealed higher cell intrinsic PD-1 expression in these cell lines which may have implications for tumor behavior and potential treatment opportunities in the future. Further work is necessary to determine the expression patterns in canine UC and potential for benefit with immunotherapy directed against PD-1 and PD-L1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Carcinoma de Células Transicionales / Antígeno B7-H1 / Receptor de Muerte Celular Programada 1 Límite: Animals Idioma: En Revista: Vet Immunol Immunopathol Año: 2022 Tipo del documento: Article Pais de publicación: Países Bajos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Carcinoma de Células Transicionales / Antígeno B7-H1 / Receptor de Muerte Celular Programada 1 Límite: Animals Idioma: En Revista: Vet Immunol Immunopathol Año: 2022 Tipo del documento: Article Pais de publicación: Países Bajos