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Impact of mixed lignocellulosic substrate and fungal consortia to enhance cellulase production and its application in NiFe2O4 nanoparticles mediated enzymatic hydrolysis of wheat straw.
Srivastava, Neha; Singh, Rajeev; Srivastava, Manish; Syed, Asad; Bahadur Pal, Dan; Bahkali, Ali H; Mishra, P K; Gupta, Vijai Kumar.
Afiliación
  • Srivastava N; Department of Chemical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India.
  • Singh R; Department of Environmental Studies, Satyawati College, University of Delhi, Delhi 110052, India.
  • Srivastava M; Department of Chemical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India.
  • Syed A; Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia.
  • Bahadur Pal D; Department of Chemical Engineering, Birla Institute of Technology, Mesra, Ranchi 835215, Jharkhand, India.
  • Bahkali AH; Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh 11451, Saudi Arabia.
  • Mishra PK; Department of Chemical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi 221005, India.
  • Gupta VK; Biorefiningand Advanced Materials Research Center, SRUC, Kings Buildings, West Mains Road, Edinburgh EH9 3JG, UK; Centerfor Safe and Improved Food, SRUC, Kings Buildings, West Mains Road, Edinburgh EH9 3JG, UK. Electronic address: vijai.gupta@sruc.ac.uk.
Bioresour Technol ; 345: 126560, 2022 Feb.
Article en En | MEDLINE | ID: mdl-34915113
Economic biowaste to biofuels production technology suffers from issues including high production cost of cellulase enzyme and its low efficiency. In this study five lignocellulosic biomass based on their high cellulosic contents are employed in 1:1 ratio with mixed fungal consortia to achieve enhance cellulase production via solid state fermentation. Under the optimum condition total 41 IU/gds FP activity was achieved in 120 h at 40 °C and pH 6.0. Further, crude cellulase was evaluated to improve thermal and pH stability under the influence of 2.0 mg/L NiFe2O4 nanoparticles, showed stability at 70 °C and pH 6.0 up to 8 h. Consequently, NiFe2O4 nanoparticles treated cellulase was used for the enzymatic hydrolysis of alkali treated wheat straw, and total 53 g/L reducing sugars could be produced in 18 h at 65 °C and pH 6.0. Thus, nanoparticles mediated enzymatic hydrolysis exhibited âˆ¼ 29% and âˆ¼ 28% higher sugar yield and productivity as compared to control after 18 h.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Celulasa / Nanopartículas Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Celulasa / Nanopartículas Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido