Your browser doesn't support javascript.
loading
Stimulated Raman histology in the neurosurgical workflow of a major European neurosurgical center - part A.
Neidert, Nicolas; Straehle, Jakob; Erny, Daniel; Sacalean, Vlad; El Rahal, Amir; Steybe, David; Schmelzeisen, Rainer; Vlachos, Andreas; Reinacher, Peter Christoph; Coenen, Volker Arnd; Mizaikoff, Boris; Heiland, Dieter Henrik; Prinz, Marco; Beck, Jürgen; Schnell, Oliver.
Afiliación
  • Neidert N; Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.
  • Straehle J; Microenvironment and Immunology Research Laboratory, Medical Center, University of Freiburg, Freiburg, Germany.
  • Erny D; Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.
  • Sacalean V; Institute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
  • El Rahal A; Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.
  • Steybe D; Microenvironment and Immunology Research Laboratory, Medical Center, University of Freiburg, Freiburg, Germany.
  • Schmelzeisen R; Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.
  • Vlachos A; Department of Oral and Maxillofacial Surgery, Medical Center, University of Freiburg, Freiburg, Germany.
  • Reinacher PC; Department of Oral and Maxillofacial Surgery, Medical Center, University of Freiburg, Freiburg, Germany.
  • Coenen VA; Medical Faculty, Freiburg University, Freiburg, Germany.
  • Mizaikoff B; Medical Faculty, Freiburg University, Freiburg, Germany.
  • Heiland DH; Department of Neuroanatomy, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
  • Prinz M; Center for Basics in NeuroModulation (NeuroModulBasics), Faculty of Medicine, University of Freiburg, Freiburg, Germany.
  • Beck J; Center Brain Links Brain Tools, University of Freiburg, Freiburg, Germany.
  • Schnell O; Medical Faculty, Freiburg University, Freiburg, Germany.
Neurosurg Rev ; 45(2): 1731-1739, 2022 Apr.
Article en En | MEDLINE | ID: mdl-34914024
Histopathological diagnosis is the current standard for the classification of brain and spine tumors. Raman spectroscopy has been reported to allow fast and easy intraoperative tissue analysis. Here, we report data on the intraoperative implementation of a stimulated Raman histology (SRH) as an innovative strategy offering intraoperative near real-time histopathological analysis. A total of 429 SRH images from 108 patients were generated and analyzed by using a Raman imaging system (Invenio Imaging Inc.). We aimed at establishing a dedicated workflow for SRH serving as an intraoperative diagnostic, research, and quality control tool in the neurosurgical operating room (OR). First experiences with this novel imaging modality were reported and analyzed suggesting process optimization regarding tissue collection, preparation, and imaging. The Raman imaging system was rapidly integrated into the surgical workflow of a large neurosurgical center. Within a few minutes of connecting the device, the first high-quality images could be acquired in a "plug-and-play" manner. We did not encounter relevant obstacles and the learning curve was steep. However, certain prerequisites regarding quality and acquisition of tissue samples, data processing and interpretation, and high throughput adaptions must be considered. Intraoperative SRH can easily be integrated into the workflow of neurosurgical tumor resection. Considering few process optimizations that can be implemented rapidly, high-quality images can be obtained near real time. Hence, we propose SRH as a complementary tool for the diagnosis of tumor entity, analysis of tumor infiltration zones, online quality and safety control and as a research tool in the neurosurgical OR.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Encefálicas Límite: Humans Idioma: En Revista: Neurosurg Rev Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Encefálicas Límite: Humans Idioma: En Revista: Neurosurg Rev Año: 2022 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania