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High-resolution relaxometry-based calibrated fMRI in murine brain: Metabolic differences between awake and anesthetized states.
Xu, Mengyang; Bo, Binshi; Pei, Mengchao; Chen, Yuyan; Shu, Christina Y; Qin, Qikai; Hirschler, Lydiane; Warnking, Jan M; Barbier, Emmanuel L; Wei, Zhiliang; Lu, Hanzhang; Herman, Peter; Hyder, Fahmeed; Liu, Zhi-Jie; Liang, Zhifeng; Thompson, Garth J.
Afiliación
  • Xu M; iHuman Institute, ShanghaiTech University, Shanghai, China.
  • Bo B; School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
  • Pei M; Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
  • Chen Y; University of Chinese Academy of Sciences, Beijing, China.
  • Shu CY; CAS Center for Excellence in Brain Sciences and Intelligence Technology, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China.
  • Qin Q; CAS Center for Excellence in Brain Sciences and Intelligence Technology, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China.
  • Hirschler L; CAS Center for Excellence in Brain Sciences and Intelligence Technology, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China.
  • Warnking JM; Biomedical Engineering, Yale University, New Haven, CT, USA.
  • Barbier EL; Magnetic Resonance Research Center (MRRC), Yale University, New Haven, CT, USA.
  • Wei Z; iHuman Institute, ShanghaiTech University, Shanghai, China.
  • Lu H; School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
  • Herman P; Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
  • Hyder F; Grenoble Institut des Neurosciences, Inserm, Univ. Grenoble Alpes, Grenoble, France.
  • Liu ZJ; C.J. Gorter Center for High Field MRI, Department of Radiology, Leiden University Medical Center, Leiden, The Netherlands.
  • Liang Z; Grenoble Institut des Neurosciences, Inserm, Univ. Grenoble Alpes, Grenoble, France.
  • Thompson GJ; Grenoble Institut des Neurosciences, Inserm, Univ. Grenoble Alpes, Grenoble, France.
J Cereb Blood Flow Metab ; 42(5): 811-825, 2022 05.
Article en En | MEDLINE | ID: mdl-34910894
Functional magnetic resonance imaging (fMRI) techniques using the blood-oxygen level-dependent (BOLD) signal have shown great potential as clinical biomarkers of disease. Thus, using these techniques in preclinical rodent models is an urgent need. Calibrated fMRI is a promising technique that can provide high-resolution mapping of cerebral oxygen metabolism (CMRO2). However, calibrated fMRI is difficult to use in rodent models for several reasons: rodents are anesthetized, stimulation-induced changes are small, and gas challenges induce noisy CMRO2 predictions. We used, in mice, a relaxometry-based calibrated fMRI method which uses cerebral blood flow (CBF) and the BOLD-sensitive magnetic relaxation component, R2', the same parameter derived in the deoxyhemoglobin-dilution model of calibrated fMRI. This method does not use any gas challenges, which we tested on mice in both awake and anesthetized states. As anesthesia induces a whole-brain change, our protocol allowed us to overcome the former limitations of rodent studies using calibrated fMRI. We revealed 1.5-2 times higher CMRO2, dependent upon brain region, in the awake state versus the anesthetized state. Our results agree with alternative measurements of whole-brain CMRO2 in the same mice and previous human anesthesia studies. The use of calibrated fMRI in rodents has much potential for preclinical fMRI.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vigilia / Imagen por Resonancia Magnética Tipo de estudio: Guideline / Prognostic_studies Límite: Animals Idioma: En Revista: J Cereb Blood Flow Metab Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vigilia / Imagen por Resonancia Magnética Tipo de estudio: Guideline / Prognostic_studies Límite: Animals Idioma: En Revista: J Cereb Blood Flow Metab Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos