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Enzymatic glucosylation of polyphenols using glucansucrases and branching sucrases of glycoside hydrolase family 70.
Li, Xiaodan; Meng, Xiangfeng; de Leeuw, Tijn C; Te Poele, Evelien M; Pijning, Tjaard; Dijkhuizen, Lubbert; Liu, Weifeng.
Afiliación
  • Li X; College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, People's Republic of China.
  • Meng X; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, People's Republic of China.
  • de Leeuw TC; CarbExplore Research BV, Groningen, The Netherlands.
  • Te Poele EM; CarbExplore Research BV, Groningen, The Netherlands.
  • Pijning T; Biomolecular X-ray Crystallography, University of Groningen, Groningen, The Netherlands.
  • Dijkhuizen L; CarbExplore Research BV, Groningen, The Netherlands.
  • Liu W; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, People's Republic of China.
Crit Rev Food Sci Nutr ; 63(21): 5247-5267, 2023.
Article en En | MEDLINE | ID: mdl-34907830
Polyphenols exhibit various beneficial biological activities and represent very promising candidates as active compounds for food industry. However, the low solubility, poor stability and low bioavailability of polyphenols have severely limited their industrial applications. Enzymatic glycosylation is an effective way to improve the physicochemical properties of polyphenols. As efficient transglucosidases, glycoside hydrolase family 70 (GH70) glucansucrases naturally catalyze the synthesis of polysaccharides and oligosaccharides from sucrose. Notably, GH70 glucansucrases show broad acceptor substrate promiscuity and catalyze the glucosylation of a wide range of non-carbohydrate hydroxyl group-containing molecules, including benzenediol, phenolic acids, flavonoids and steviol glycosides. Branching sucrase enzymes, a newly established subfamily of GH70, are shown to possess a broader acceptor substrate binding pocket that acts efficiently for glucosylation of larger size polyphenols such as flavonoids. Here we present a comprehensive review of glucosylation of polyphenols using GH70 glucansucrase and branching sucrases. Their catalytic efficiency, the regioselectivity of glucosylation and the structure of generated products are described for these reactions. Moreover, enzyme engineering is effective for improving their catalytic efficiency and product specificity. The combined information provides novel insights on the glucosylation of polyphenols by GH70 glucansucrases and branching sucrases, and may promote their applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polifenoles / Glicósido Hidrolasas Idioma: En Revista: Crit Rev Food Sci Nutr Asunto de la revista: CIENCIAS DA NUTRICAO Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polifenoles / Glicósido Hidrolasas Idioma: En Revista: Crit Rev Food Sci Nutr Asunto de la revista: CIENCIAS DA NUTRICAO Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos