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The effects of plasma-derived extracellular vesicles on cumulus expansion and oocyte maturation in mice.
Javadi, Maryam; Soleimani Rad, Jafar; Pashaiasl, Maryam; Farashah, Mohammad Sadegh Gholami; Roshangar, Leila.
Afiliación
  • Javadi M; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Soleimani Rad J; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Pashaiasl M; Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; Women's Reproductive Health Research Center, Tabriz University o
  • Farashah MSG; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Roshangar L; Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: lroshangar@yahoo.com.
Reprod Biol ; 22(1): 100593, 2022 Mar.
Article en En | MEDLINE | ID: mdl-34906824
Cumulus cell expansion is required for the ovulation of a fertilizable oocyte. Extracellular vesicles (EVs) are bilayer-lipid membrane vesicles that may be found in a variety of bodily fluids and play an important role in biological processes. This study aimed to examine the effects of plasma-derived EVs on cumulus expansion and in vitro maturation (IVM) of the oocyte. EVswere isolated using ultracentrifugation from the plasma of female mice. The morphology and size of EVs were analyzed by transmission electron microscopy (TEM) and dynamic light scattering (DLS). Western blotting allowed us to identify CD63, CD81, CD9, and HSP70 protein markers of EVs; the expression of the genes related to cumulus cell expansion, including hyaluronan synthase 2 (Has2) and prostaglandinendoperoxide synthase 2 (Ptgs2), were assessed using real-time polymerase chain reaction. Plasma-derived EVs labeled with Dil dye were successfully incorporated with cumulus cells during IVM. Plasma-derived EVs significantly induced cumulus expansion and maturation of oocytes. The percentage of oocytes that reached the MII stage was significantly greater in the EVs treatment group compared with other groups. Although treatment with epidermal growth factor (EGF) significantly increased cumulus expansion in cumulus-oocyte complexes (COCs), the impact was less than that seen with plasma-derived EVs. Furthermore, EVs generated from plasma substantially enhanced Has2 and Ptgs2 mRNA expression in the cumulus-oocyte complex. This research indicates that EVs derived from plasma are capable of promoting cumulus expansion and oocyte maturation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células del Cúmulo / Vesículas Extracelulares Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Reprod Biol Asunto de la revista: MEDICINA REPRODUTIVA Año: 2022 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Polonia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células del Cúmulo / Vesículas Extracelulares Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Reprod Biol Asunto de la revista: MEDICINA REPRODUTIVA Año: 2022 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Polonia