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Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis.
Chen, Cheng-Cheung; Chen, Yih-Yuan; Yeh, Chang-Ching; Hsu, Chia-Wei; Yu, Shang-Jie; Hsu, Chih-Hao; Wei, Ting-Chun; Ho, Sin-Ni; Tsai, Pei-Chu; Song, Yung-Deng; Yen, Hui-Ju; Chen, Xin-An; Young, Jenn-Jong; Chuang, Chuan-Chung; Dou, Horng-Yunn.
Afiliación
  • Chen CC; Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan.
  • Chen YY; Graduate Institute of Medical Science, National Defense Medical Center, Taipei, Taiwan.
  • Yeh CC; Department of Biochemical Science and Technology, National Chiayi University, Chia-Yi, Taiwan.
  • Hsu CW; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
  • Yu SJ; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
  • Hsu CH; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
  • Wei TC; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
  • Ho SN; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
  • Tsai PC; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
  • Song YD; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
  • Yen HJ; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, Taiwan.
  • Chen XA; Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan.
  • Young JJ; School of Pharmacy, National Defense Medical Center, Taipei, Taiwan.
  • Chuang CC; Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan.
  • Dou HY; Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan.
Front Pharmacol ; 12: 746496, 2021.
Article en En | MEDLINE | ID: mdl-34899300
Tuberculosis (TB) is a leading cause of death from a single infectious agent, Mycobacterium tuberculosis (Mtb). Although progress has been made in TB control, still about 10 million people worldwide develop TB annually and 1.5 million die of the disease. The rapid emergence of aggressive, drug-resistant strains and latent infections have caused TB to remain a global health challenge. TB treatments are lengthy and their side effects lead to poor patient compliance, which in turn has contributed to the drug resistance and exacerbated the TB epidemic. The relatively low output of newly approved antibiotics has spurred research interest toward alternative antibacterial molecules such as silver nanoparticles (AgNPs). In the present study, we use the natural biopolymer alginate to serve as a stabilizer and/or reductant to green synthesize AgNPs, which improves their biocompatibility and avoids the use of toxic chemicals. The average size of the alginate-capped AgNPs (ALG-AgNPs) was characterized as nanoscale, and the particles were round in shape. Drug susceptibility tests showed that these ALG-AgNPs are effective against both drug-resistant Mtb strains and dormant Mtb. A bacterial cell-wall permeability assay showed that the anti-mycobacterial action of ALG-AgNPs is mediated through an increase in cell-wall permeability. Notably, the anti-mycobacterial potential of ALG-AgNPs was effective in both zebrafish and mouse TB animal models in vivo. These results suggest that ALG-AgNPs could provide a new therapeutic option to overcome the difficulties of current TB treatments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Pharmacol Año: 2021 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Pharmacol Año: 2021 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza