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Molecular Activation of the Kv11.1 Channel Reprograms EMT in Colon Cancer by Inhibiting TGFß Signaling via Activation of Calcineurin.
Eskandari, Najmeh; Senyuk, Vitalyi; Moore, Jennifer; Kalik, Zane; Luan, Qiyue; Papautsky, Ian; Moshiri, Arfa; Bocchetta, Maurizio; Salami, Seyed Alireza; Oryan, Shahrbanoo; Gentile, Saverio.
Afiliación
  • Eskandari N; Division of Hematology Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
  • Senyuk V; Department of Animal Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran 15719-14911, Iran.
  • Moore J; Division of Hematology Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
  • Kalik Z; Division of Hematology Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
  • Luan Q; Division of Hematology Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
  • Papautsky I; Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60612, USA.
  • Moshiri A; Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60612, USA.
  • Bocchetta M; Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran 19857-17413, Iran.
  • Salami SA; Microbiology Research Center, Pasteur Institute of Iran, Tehran 13169-43551, Iran.
  • Oryan S; Cardinal Bernardin Cancer Center, Department of Cancer Biology, Loyola University Chicago, Maywood, IL 60153, USA.
  • Gentile S; Department of Biotechnology, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj 31587-77871, Iran.
Cancers (Basel) ; 13(23)2021 Nov 30.
Article en En | MEDLINE | ID: mdl-34885136
Control of ionic gradients is critical to maintain cellular homeostasis in both physiological and pathological conditions, but the role of ion channels in cancer cells has not been studied thoroughly. In this work we demonstrated that activity of the Kv11.1 potassium channel plays a vital role in controlling the migration of colon cancer cells by reversing the epithelial-to-mesenchymal transition (EMT) into the mesenchymal-to-epithelial transition (MET). We discovered that pharmacological stimulation of the Kv11.1 channel with the activator molecule NS1643 produces a strong inhibition of colon cancer cell motility. In agreement with the reversal of EMT, NS1643 treatment leads to a depletion of mesenchymal markers such as SNAIL1, SLUG, TWIST, ZEB, N-cadherin, and c-Myc, while the epithelial marker E-cadherin was strongly upregulated. Investigating the mechanism linking Kv11.1 activity to reversal of EMT into MET revealed that stimulation of Kv11.1 produced a strong and fast inhibition of the TGFß signaling. Application of NS1643 resulted in de-phosphorylation of the TGFß downstream effectors R-SMADs by activation of the serine/threonine phosphatase PP2B (calcineurin). Consistent with the role of TGFß in controlling cancer stemness, NS1643 also produced a strong inhibition of NANOG, SOX2, and OCT4 while arresting the cell cycle in G0/G1. Our data demonstrate that activation of the Kv11.1 channel reprograms EMT into MET by inhibiting TGFß signaling, which results in inhibition of motility in colon cancer cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Cancers (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Cancers (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza