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New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.
Yermak, Irina; Anastyuk, Stanislav; Kravchenko, Anna; Helbert, William; Glazunov, Valery; Shulgin, Andrey; Spirin, Pavel; Prassolov, Vladimir.
Afiliación
  • Yermak I; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russia.
  • Anastyuk S; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russia.
  • Kravchenko A; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russia.
  • Helbert W; CERMAV, CNRS and Grenoble Alpes Université, BP53, CEDEX 09, 38000 Grenoble, France.
  • Glazunov V; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022 Vladivostok, Russia.
  • Shulgin A; Engelhardt-Institute of Molecular Biology, Russian Academy of Sciences, Vavilova, 32, 119991 Moscow, Russia.
  • Spirin P; Moscow Institute of Physics and Technology (State University), Institutskiy per., 9, Dolgoprudny, 141701 Moscow, Russia.
  • Prassolov V; Engelhardt-Institute of Molecular Biology, Russian Academy of Sciences, Vavilova, 32, 119991 Moscow, Russia.
Int J Mol Sci ; 22(23)2021 Nov 29.
Article en En | MEDLINE | ID: mdl-34884718
New insights into the structure of the hybrid κ/ß-carrageenan (κ/ß-CRG) of the red alga Tichocarpus crinitus have been obtained. Carrageenan oligosaccharides were prepared through the chemical and enzymatic depolymerization of κ/ß-CRG with κ-carrageenase and its the enzyme-resistant fraction. The composition and distribution of the repetition units of κ/ß- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide. An analysis revealed the blockwise distribution of the long ß-blocks along the polysaccharide chain, with the inclusion of κ/ß, µ/ν-blocks and some ι-blocks. Furthermore, the desulfated κ/ß-CRG was shown to contain of -G-D- repeating units up to 3.5 kDa. Previous studies have demonstrated that CRGs suppress the replication of several viruses. Here, we established that κ/ß-CRG and its oligosaccharides significantly inhibit the transduction efficiency of replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses. We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells. The CRG oligosaccharides displayed significantly higher antiviral activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antivirales / Carragenina / Infecciones por VIH / VIH-1 / Lentivirus / Proteínas gp160 de Envoltorio del VIH Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antivirales / Carragenina / Infecciones por VIH / VIH-1 / Lentivirus / Proteínas gp160 de Envoltorio del VIH Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza