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Spatial trans-epithelial electrical resistance (S-TEER) integrated in organs-on-chips.
Renous, Noa; Kiri, Mark D; Barnea, Ronny A; Rauti, Rossana; Leichtmann-Bardoogo, Yael; Maoz, Ben M.
Afiliación
  • Renous N; Department of Biomedical Engineering, Tel Aviv University, Tel Aviv 69978, Israel. bmaoz@tauex.tau.ac.il.
  • Kiri MD; Department of Biomedical Engineering, Tel Aviv University, Tel Aviv 69978, Israel. bmaoz@tauex.tau.ac.il.
  • Barnea RA; School of Chemistry, Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
  • Rauti R; Department of Biomedical Engineering, Tel Aviv University, Tel Aviv 69978, Israel. bmaoz@tauex.tau.ac.il.
  • Leichtmann-Bardoogo Y; Department of Biomedical Engineering, Tel Aviv University, Tel Aviv 69978, Israel. bmaoz@tauex.tau.ac.il.
  • Maoz BM; Department of Biomedical Engineering, Tel Aviv University, Tel Aviv 69978, Israel. bmaoz@tauex.tau.ac.il.
Lab Chip ; 22(1): 71-79, 2021 12 21.
Article en En | MEDLINE | ID: mdl-34878455
Transepithelial/transendothelial electrical resistance (TEER) is a label-free assay that is commonly used to assess tissue barrier integrity. TEER measurement systems have been embedded in organ-on-a-chip devices to provide live readouts of barrier functionality. Yet, these systems commonly provide the impedance values which correspond to the highest level of permeability throughout the chip and cannot provide localized information on specific regions of interest. This work introduces a system that provides this essential information: a spatial-TEER (S-TEER) organ-on-a-chip platform, which incorporates moving (scanning) electrodes that can measure electrical resistance at any desired location along the chip. We demonstrate the system's capacity to obtain localized measurements of permeability in selected regions of a cell sample. We show how, in a layer with non-uniform levels of cell coverage, permeability is higher in areas with lower cell density-suggesting that the system can be used to monitor local cellular growth in vitro. To demonstrate the applicability of the chip in studies of barrier function, we characterize tissue response to TNF-α and to EGTA, agents known to harm tissue barrier integrity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dispositivos Laboratorio en un Chip Idioma: En Revista: Lab Chip Asunto de la revista: BIOTECNOLOGIA / QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dispositivos Laboratorio en un Chip Idioma: En Revista: Lab Chip Asunto de la revista: BIOTECNOLOGIA / QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Reino Unido