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A validated method for the separation of ethyl glucoside isomers by gas chromatography-tandem mass spectrometry and quantitation in human whole blood and urine.
Waters, Brian; Nakano, Ryoko; Hara, Kenji; Matsusue, Aya; Kashiwagi, Masayuki; Kubo, Shin-Ichi.
Afiliación
  • Waters B; Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Fukuoka, Japan. Electronic address: bwaters@fukuoka-u.ac.jp.
  • Nakano R; Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Fukuoka, Japan; Department of Anesthesiology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
  • Hara K; Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
  • Matsusue A; Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
  • Kashiwagi M; Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
  • Kubo SI; Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1188: 123074, 2022 Jan 01.
Article en En | MEDLINE | ID: mdl-34875494
Ethyl glucoside (EG) is present in Japanese sake in high concentrations, and can be found in other alcoholic beverages like beer and wine in varying amounts. EG exists as alpha (α) and beta (ß) isomers, and the concentrations and ratios of these isomers differ depending on the alcoholic beverage. Herein, we report a validated analysis method for the separation of EG isomers in human whole blood and urine, by GC-MS/MS. Whole blood and urine samples were deproteinized and interferences removed by weak cation exchange cartridges. The target analytes were acetylated using acetic anhydride and pyridine by microwave-accelerated derivatization. Separation was performed using tandem columns, with detection in the multiple reaction monitoring (MRM) mode. The MRM transitions for all compounds were m/z 157.0 > 115.1 for the quantifying transition, and m/z 157.0 > 73.1 and m/z 141.0 > 81.0 for the qualifying transitions. Assay validation included linearity, LOD and LLOQ, bias, within-run and between-run precision, stability, and dilution integrity. Baseline separation of the 2 isomers was achieved with linear calibration (r2 > 0.99) across the calibration range 0.625 to 50 µg/mL for both α- and ß-EG in both whole blood and urine. The validated method was then applied to actual human whole blood and urine samples collected at autopsy, as well as relevant alcoholic beverage samples. The quantitation of EG isomers could benefit the forensic toxicology community by acting as markers for recent alcoholic beverage consumption.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas en Tándem / Glucósidos / Cromatografía de Gases y Espectrometría de Masas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas en Tándem / Glucósidos / Cromatografía de Gases y Espectrometría de Masas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2022 Tipo del documento: Article Pais de publicación: Países Bajos