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Rapid test to assess the escape of SARS-CoV-2 variants of concern.
Heggestad, Jacob T; Britton, Rhett J; Kinnamon, David S; Wall, Simone A; Joh, Daniel Y; Hucknall, Angus M; Olson, Lyra B; Anderson, Jack G; Mazur, Anna; Wolfe, Cameron R; Oguin, Thomas H; Sullenger, Bruce A; Burke, Thomas W; Kraft, Bryan D; Sempowski, Gregory D; Woods, Christopher W; Chilkoti, Ashutosh.
Afiliación
  • Heggestad JT; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708, USA.
  • Britton RJ; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708, USA.
  • Kinnamon DS; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708, USA.
  • Wall SA; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708, USA.
  • Joh DY; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708, USA.
  • Hucknall AM; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708, USA.
  • Olson LB; Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, NC 27710, USA.
  • Anderson JG; Center for Applied Genomics and Precision Medicine, Department of Medicine, Duke University, Durham, NC 27710, USA.
  • Mazur A; Center for Applied Genomics and Precision Medicine, Department of Medicine, Duke University, Durham, NC 27710, USA.
  • Wolfe CR; Division of Infectious Diseases, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.
  • Oguin TH; Department of Medicine and the Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
  • Sullenger BA; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708, USA.
  • Burke TW; Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, NC 27710, USA.
  • Kraft BD; Department of Surgery, Duke University School of Medicine, Durham, NC 27710, USA.
  • Sempowski GD; Center for Applied Genomics and Precision Medicine, Department of Medicine, Duke University, Durham, NC 27710, USA.
  • Woods CW; Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.
  • Chilkoti A; Department of Medicine and the Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Sci Adv ; 7(49): eabl7682, 2021 Dec 03.
Article en En | MEDLINE | ID: mdl-34860546
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants are concerning in the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here, we developed a rapid test, termed CoVariant-SCAN, that detects neutralizing antibodies (nAbs) capable of blocking interactions between the angiotensin-converting enzyme 2 receptor and the spike protein of wild-type (WT) SARS-CoV-2 and three other variants: B.1.1.7, B.1.351, and P.1. Using CoVariant-SCAN, we assessed neutralization/blocking of monoclonal antibodies and plasma from COVID-19­positive and vaccinated individuals. For several monoclonal antibodies and most plasma samples, neutralization against B.1.351 and P.1 variants is diminished relative to WT, while B.1.1.7 is largely cross-neutralized. We also showed that we can rapidly adapt the platform to detect nAbs against an additional variant­B.1.617.2 (Delta)­without reengineering or reoptimizing the assay. Results using CoVariant-SCAN are consistent with live virus neutralization assays and demonstrate that this easy-to-deploy test could be used to rapidly assess nAb response against multiple SARS-CoV-2 variants.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Sci Adv Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Sci Adv Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos