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Tonic Serotonin Measurements In Vivo Using N-Shaped Multiple Cyclic Square Wave Voltammetry.
Shin, Hojin; Goyal, Abhinav; Barnett, J Hudson; Rusheen, Aaron E; Yuen, Jason; Jha, Rohan; Hwang, Sang Mun; Kang, Yumin; Park, Cheonho; Cho, Hyun-U; Blaha, Charles D; Bennet, Kevin E; Oh, Yoonbae; Heien, Michael L; Jang, Dong Pyo; Lee, Kendall H.
Afiliación
  • Shin H; Department of Neurologic Surgery, Mayo Clinic, Rochester, Minnesota 55905, United States.
  • Goyal A; Department of Neurologic Surgery, Mayo Clinic, Rochester, Minnesota 55905, United States.
  • Barnett JH; Medical Scientist Training Program, Mayo Clinic, Rochester, Minnesota 55905, United States.
  • Rusheen AE; Department of Neurologic Surgery, Mayo Clinic, Rochester, Minnesota 55905, United States.
  • Yuen J; Medical Scientist Training Program, Mayo Clinic, Rochester, Minnesota 55905, United States.
  • Jha R; Department of Neurologic Surgery, Mayo Clinic, Rochester, Minnesota 55905, United States.
  • Hwang SM; Medical Scientist Training Program, Mayo Clinic, Rochester, Minnesota 55905, United States.
  • Kang Y; Department of Neurologic Surgery, Mayo Clinic, Rochester, Minnesota 55905, United States.
  • Park C; Deakin University, IMPACT - The Institute for Mental and Physical Health and Clinical Translation, School of Medicine, Barwon Health, Geelong, VIC 3216, Australia.
  • Cho HU; Department of Neurologic Surgery, Mayo Clinic, Rochester, Minnesota 55905, United States.
  • Blaha CD; Department of Biomedical Engineering, Hanyang University, Seoul 04763, Republic of Korea.
  • Bennet KE; Department of Biomedical Engineering, Hanyang University, Seoul 04763, Republic of Korea.
  • Oh Y; Department of Biomedical Engineering, Hanyang University, Seoul 04763, Republic of Korea.
  • Heien ML; Department of Biomedical Engineering, Hanyang University, Seoul 04763, Republic of Korea.
  • Jang DP; Department of Neurologic Surgery, Mayo Clinic, Rochester, Minnesota 55905, United States.
  • Lee KH; Department of Neurologic Surgery, Mayo Clinic, Rochester, Minnesota 55905, United States.
Anal Chem ; 93(51): 16987-16994, 2021 12 28.
Article en En | MEDLINE | ID: mdl-34855368
Here, we present the development of a novel voltammetric technique, N-shaped multiple cyclic square wave voltammetry (N-MCSWV) and its application in vivo. It allows quantitative measurements of tonic extracellular levels of serotonin in vivo with mitigated fouling effects. N-MCSWV enriches the electrochemical information by generating high dimensional voltammograms, which enables high sensitivity and selectivity against 5-hydroindoleacetic acid (5-HIAA), dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC), histamine, ascorbic acid, norepinephrine, adenosine, and pH. Using N-MCSWV, in combination with PEDOT:Nafion-coated carbon fiber microelectrodes, a tonic serotonin concentration of 52 ± 5.8 nM (n = 20 rats, ±SEM) was determined in the substantia nigra pars reticulata of urethane-anesthetized rats. Pharmacological challenges with dopaminergic, noradrenergic, and serotonergic synaptic reuptake inhibitors supported the ability of N-MCSWV to selectively detect tonic serotonin levels in vivo. Overall, N-MCSWV is a novel voltammetric technique for analytical quantification of serotonin. It offers continuous monitoring of changes in tonic serotonin concentrations in the brain to further our understanding of the role of serotonin in normal behaviors and psychiatric disorders.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dopamina / Serotonina Límite: Animals Idioma: En Revista: Anal Chem Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dopamina / Serotonina Límite: Animals Idioma: En Revista: Anal Chem Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos