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Fungicide resistance characterized across seven modes of action in Botrytis cinerea isolated from Australian vineyards.
Harper, Lincoln A; Paton, Scott; Hall, Barbara; McKay, Suzanne; Oliver, Richard P; Lopez-Ruiz, Francisco J.
Afiliación
  • Harper LA; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, Australia.
  • Paton S; Nufarm, Kwinana Beach, Australia.
  • Hall B; South Australian Research and Development Institute, Plant Health and Biosecurity, Urrbrae, Australia.
  • McKay S; South Australian Research and Development Institute, Plant Health and Biosecurity, Urrbrae, Australia.
  • Oliver RP; School of Molecular and Life Sciences, Curtin University, Bentley, Australia.
  • Lopez-Ruiz FJ; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, Australia.
Pest Manag Sci ; 78(4): 1326-1340, 2022 Apr.
Article en En | MEDLINE | ID: mdl-34854539
BACKGROUND: Botrytis bunch rot, caused by Botrytis cinerea, is an economically important disease of grapes in Australia and across grape-growing regions worldwide. Control of this disease relies on canopy management and the application of fungicides. Fungicide application can lead to the selection of resistant B. cinerea populations, which has an adverse effect on the management of the disease. Characterizing the distribution and severity of resistant B. cinerea populations is needed to inform resistance management strategies. RESULTS: In this study, 724 isolates were sampled from 76 Australian vineyards during 2013-2016 and were screened against seven fungicides with different modes of action (MOAs). The resistance frequencies for azoxystrobin, boscalid, fenhexamid, fludioxonil, iprodione, pyrimethanil and tebuconazole were 5%, 2.8%, 2.1%, 6.2%, 11.6%, 7.7% and 2.9%, respectively. Nearly half of the resistant isolates (43.8%) were resistant to more than one of the fungicides tested. The frequency of vineyards with at least one isolate simultaneously resistant to one, two, three, four or five fungicides was 19.7%, 7.9%, 6.6%, 10.5% and 2.6%. Resistance was associated with previously published genotypes in CytB (G143A), SdhB (H272R/Y), Erg27 (F412S), Mrr1 (D354Y), Bos1 (I365S, N373S + Q369P, I365S + D757N) and Pos5 (V273I, P319A, L412F/V). Novel genotypes were also described in Mrr1 (S611N, D616G), Pos5 (V273L) and Cyp51 (P347S). Expression analysis was used to characterize fludioxonil-resistant isolates exhibiting overexpression (6.3-9.6-fold) of the ABC transporter gene AtrB (MDR1 phenotype). CONCLUSION: Resistance frequencies were lower when compared to most previously published surveys of B. cinerea resistance in grape and other crops. Nevertheless, continued monitoring of critical MOAs used in Australian vineyards is recommended. © 2021 Society of Chemical Industry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Botrytis / Fungicidas Industriales País/Región como asunto: Oceania Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Botrytis / Fungicidas Industriales País/Región como asunto: Oceania Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido