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Follicle-stimulating hormone mediates the consumption of serum-derived glycogen by bovine cumulus-oocyte complexes during in vitro maturation.
Cantanhêde, Ludymila F; Santos-Silva, Cristiane T; Moura, Marcelo T; Ferreira-Silva, José C; Oliveira, Júnior M B; Gonçalves, Daniel N A; Teixeira, Álvaro A C; Wanderley-Teixeira, Valéria; Oliveira, Marcos A L.
Afiliación
  • Cantanhêde LF; Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife 52171900, Brazil.
  • Santos-Silva CT; Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco - UFRPE, Recife 52171900, Brazil.
  • Moura MT; Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife 52171900, Brazil.
  • Ferreira-Silva JC; Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife 52171900, Brazil.
  • Oliveira JMB; Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife 52171900, Brazil.
  • Gonçalves DNA; Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife 52171900, Brazil.
  • Teixeira ÁAC; Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco - UFRPE, Recife 52171900, Brazil.
  • Wanderley-Teixeira V; Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco - UFRPE, Recife 52171900, Brazil.
  • Oliveira MAL; Department of Veterinary Medicine, Federal Rural University of Pernambuco - UFRPE, Recife 52171900, Brazil.
Vet World ; 14(9): 2512-2517, 2021 Sep.
Article en En | MEDLINE | ID: mdl-34840472
BACKGROUND AND AIM: Oocyte in vitro maturation (IVM) is an appealing approach for several assisted reproductive technologies and dissecting oocyte maturation. Nonetheless, IVM leads to lower developmental competence and usually relies on undefined, serum-containing media. Therefore, biochemical profiling aimed to explore fluctuations in IVM media content during the acquisition of oocyte developmental competence. MATERIALS AND METHODS: Bovine cumulus-oocyte complexes (COCs) underwent IVM in TCM199 medium with Earle's salts, supplemented with 2.0 mM L-glutamine, 10% fetal bovine serum, antibiotics, and 0.05 IU/mL porcine follicle-stimulating hormone (FSH+) or vehicle control (CTL) medium for 22 h. RESULTS: FSH withdrawal (CTL) diminished several processes associated with the acquisition of oocyte developmental competence, such as reduced cumulus cell expansion, diminished estradiol synthesis (FSH+: 116.0±0.0 pg/mL vs. CTL: 97.6±18.0 pg/mL), and lower oocyte nuclear maturation rate (FSH+: 96.47% vs. CTL: 88.76%). Fresh media formulations (i.e., TCM199 with FSH or vehicle) were indistinguishable under biochemical profiling threshold conditions. Biochemical profiling showed similar total protein and lipid concentrations between groups. Further, total sugar concentrations diminished from fresh media to their post-IVM counterparts, albeit in an FSH-independent manner. Glycogen concentrations remained unaltered after IVM within CTL media, albeit were substantially lower after IVM under FSH+ conditions. CONCLUSION: FSH mediates the consumption of serum-derived glycogen by bovine COCs during IVM and implies that serum-free media should contain increased glucose concentrations to facilitate the acquisition of oocyte developmental competence.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet World Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: India
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet World Año: 2021 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: India