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Comparison of RNA content from hydrophobic interaction chromatography-isolated seminal plasma exosomes from intrauterine insemination (IUI) pregnancies.
Lal, Arnav; Pike, James Frederick W; Polley, Emily L; Huang, Sisi; Sanni, Mustapha; Hailat, Tareq; Zimmerman, Shawn; Clay-Gilmour, Alyssa; Bruce, Terri F; Marcus, Kenneth R; Roudebush, William E; Chosed, Renee J.
Afiliación
  • Lal A; Department of Biomedical Sciences, University of South Carolina School of Medicine Greenville, Greenville, South Carolina, USA.
  • Pike JFW; Department of Biomedical Sciences, University of South Carolina School of Medicine Greenville, Greenville, South Carolina, USA.
  • Polley EL; Department of Biomedical Sciences, University of South Carolina School of Medicine Greenville, Greenville, South Carolina, USA.
  • Huang S; Department of Chemistry, Clemson University, Clemson, South Carolina, USA.
  • Sanni M; Department of Biomedical Sciences, University of South Carolina School of Medicine Greenville, Greenville, South Carolina, USA.
  • Hailat T; Department of Biomedical Sciences, University of South Carolina School of Medicine Greenville, Greenville, South Carolina, USA.
  • Zimmerman S; VIOS Fertility Institute, Swansea, Illinois, USA.
  • Clay-Gilmour A; Department of Epidemiology and Biostatistics, Arnold School of Public Health, University of South Carolina, Greenville, South Carolina, USA.
  • Bruce TF; Department of Bioengineering, Clemson University, Clemson, South Carolina, USA.
  • Marcus KR; Department of Chemistry, Clemson University, Clemson, South Carolina, USA.
  • Roudebush WE; Department of Biomedical Sciences, University of South Carolina School of Medicine Greenville, Greenville, South Carolina, USA.
  • Chosed RJ; Department of Biomedical Sciences, University of South Carolina School of Medicine Greenville, Greenville, South Carolina, USA.
Andrologia ; 54(2): e14325, 2022 Mar.
Article en En | MEDLINE | ID: mdl-34837240
Male factors account for roughly half of infertility cases, with most male infertility diagnosed as idiopathic. Researchers predicting intrauterine insemination success rates have identified multiple prognostic factors, including semen parameters and seminal fluid composition. Seminal plasma contains extracellular exosomes, which contain RNAs and proteins involved in spermatogenesis. The contents of seminal plasma exosomes may be an indicator of overall sperm quality or fertility potential; therefore, analysis of exosomes may provide a measure for sperm viability and fertilization potential. In our study, exosomes were isolated and purified from seminal plasma obtained from IUI treatments with known pregnancy outcomes. We used a unique method to isolate the exosomes which made use of the hydrophobic interaction chromatography method. RNASeq was performed on RNAs from the purified exosomes. This analysis revealed holistic trends, including increased expression associated with RNA originating from chromosomes 1, 10, 12, 16 and 21. Overall, total RNA was significantly decreased and rRNA was significantly increased in successful IUI attempts. Furthermore, we found specific mRNAs and lincRNAs associated with positive versus negative pregnancy outcomes. Our study isolated and purified seminal plasma exosomes without ultracentrifugation, and it provides further evidence for differences in seminal plasma exosome molecular contents associated with pregnancy status.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Exosomas / Infertilidad Masculina Tipo de estudio: Prognostic_studies Límite: Female / Humans / Male / Pregnancy Idioma: En Revista: Andrologia Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Exosomas / Infertilidad Masculina Tipo de estudio: Prognostic_studies Límite: Female / Humans / Male / Pregnancy Idioma: En Revista: Andrologia Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania