Your browser doesn't support javascript.
loading
Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway.
Zhang, Longlin; Ma, Mengmeng; Li, Zhengyi; Zhang, Haihan; He, Xi; Song, Zehe.
Afiliación
  • Zhang L; Department of Animal Science, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
  • Ma M; Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Changsha 410128, China.
  • Li Z; Department of Animal Science, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
  • Zhang H; Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Changsha 410128, China.
  • He X; Department of Animal Science, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
  • Song Z; Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Changsha 410128, China.
Molecules ; 26(22)2021 Nov 19.
Article en En | MEDLINE | ID: mdl-34834090
L-theanine is a nonprotein amino acid found in tea leaves and has been widely used as a safe food additive in beverages or foods because of its varied bioactivities. The aim of this study was to reveal the in vitro gastrointestinal protective effects of L-theanine in DSS-induced intestinal porcine enterocyte (IPEC-J2) cell models using molecular and metabolic methods. Results showed that 2.5% dextran sulfate sodium (DSS) treatment inhibited the cell proliferation of IPEC-J2 and blocked the normal operation of the cell cycle, while L-theanine pretreatment significantly preserved these trends to exert protective effects. L-theanine pre-treatment also up-regulated the EGF, CDC2, FGF2, Rb genes and down-regulated p53, p21 proliferation-related mRNA expression in DSS-treated cells, in accompany with p53 signaling pathway inhibition. Meanwhile, metabolomics analysis revealed that L-theanine and DSS treated IPEC-J2 cells have different metabolomic profiles, with significant changes in the key metabolites involved in pyrimidine metabolism and amino acid metabolism, which play an important role in nucleotide metabolism. In summary, L-theanine has a beneficial protection in DSS-induced IPEC-J2 cells via promoting proliferation and regulating metabolism disorders.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Ciclo Celular / Sulfato de Dextran / Proteína p53 Supresora de Tumor / Enterocitos / Glutamatos / Inhibidores de Crecimiento Límite: Animals Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Ciclo Celular / Sulfato de Dextran / Proteína p53 Supresora de Tumor / Enterocitos / Glutamatos / Inhibidores de Crecimiento Límite: Animals Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza