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Analysis of Schistosoma mansoni Extracellular Vesicles Surface Glycans Reveals Potential Immune Evasion Mechanism and New Insights on Their Origins of Biogenesis.
Dagenais, Maude; Gerlach, Jared Q; Wendt, George R; Collins, James J; Atkinson, Louise E; Mousley, Angela; Geary, Timothy G; Long, Thavy.
Afiliación
  • Dagenais M; Institute of Parasitology, McGill University, Ste-Anne-de-Bellevue, QC H9X 3V9, Canada.
  • Gerlach JQ; Glycoscience Group, Advanced Glycoscience Research Cluster, National Centre for Biomedical Engineering Science, National University of Ireland Galway, H91 TK33 Galway, Ireland.
  • Wendt GR; Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
  • Collins JJ; Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
  • Atkinson LE; Microbes and Pathogen Biology, The Institute for Global Food Security, School of Biological Sciences, Queen's University-Belfast, Belfast BT9 5DL, UK.
  • Mousley A; Microbes and Pathogen Biology, The Institute for Global Food Security, School of Biological Sciences, Queen's University-Belfast, Belfast BT9 5DL, UK.
  • Geary TG; Institute of Parasitology, McGill University, Ste-Anne-de-Bellevue, QC H9X 3V9, Canada.
  • Long T; Microbes and Pathogen Biology, The Institute for Global Food Security, School of Biological Sciences, Queen's University-Belfast, Belfast BT9 5DL, UK.
Pathogens ; 10(11)2021 Oct 29.
Article en En | MEDLINE | ID: mdl-34832557
Parasitic helminths are master manipulators of host immunity. Their strategy is complex and involves the release of excreted/secreted products, including extracellular vesicles (EVs). The protein and miRNA contents of EVs have been characterised for many parasitic helminths but, despite reports suggesting the importance of EV surface carbohydrate structures (glycans) in the interactions with target cells and thus subsequent effector functions, little is known about parasite EV glycomics. Using lectin microarrays, we identified several lectins that exhibit strong adhesion to Schistosoma mansoni EVs, suggesting the presence of multiple glycan structures on these vesicles. Interestingly, SNA-I, a lectin that recognises structures with terminal sialic acid, displayed strong affinity for S. mansoni EVs, which was completely abolished by neuraminidase treatment, suggesting sialylation in the EV sample. This finding is of interest, as sialic acids play important roles in the context of infection by aiding immune evasion, affecting target recognition, cell entry, etc., but are not thought to be synthesised by helminths. These data were validated by quantitative analysis of free sialic acid released from EVs following treatment with neuraminidase. Lectin histochemistry and fluorescence in situ hybridisation analyses on whole adult worms suggest the involvement of sub-tegumental cell bodies, as well as the digestive and excretory systems, in the release of EVs. These results support previous reports of EV biogenesis diversity in trematodes and potentially highlight new means of immune modulation and evasion employed by schistosomes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Pathogens Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Pathogens Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza