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Glioma Cells Acquire Stem-like Characters by Extrinsic Ribosome Stimuli.
Shirakawa, Yuki; Ohta, Kunimasa; Miyake, Shunsuke; Kanemaru, Ayumi; Kuwano, Akari; Yonemaru, Kou; Uchino, Shota; Yamaoka, Michiko; Ito, Yuki; Ito, Naofumi; Hide, Takuichiro; Shinojima, Naoki; Mukasa, Akitake; Saito, Hideyuki; Jono, Hirofumi.
Afiliación
  • Shirakawa Y; Department of Clinical Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Ohta K; Department of Stem Cell Biology, Faculty of Arts and Science, Kyushu University, Fukuoka 819-0385, Japan.
  • Miyake S; Department of Clinical Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Kanemaru A; Department of Pharmacy, Kumamoto University Hospital, Kumamoto 860-8556, Japan.
  • Kuwano A; Department of Clinical Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Yonemaru K; Department of Clinical Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Uchino S; Department of Clinical Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Yamaoka M; Department of Clinical Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Ito Y; Department of Clinical Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Ito N; Department of Clinical Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Hide T; Department of Developmental Neurobiology, Graduate School of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Shinojima N; Department of Neurosurgery, Kitasato University School of Medicine, Sagamihara 252-0375, Japan.
  • Mukasa A; Department of Neurosurgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Saito H; Department of Neurosurgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
  • Jono H; Department of Clinical Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
Cells ; 10(11)2021 11 01.
Article en En | MEDLINE | ID: mdl-34831193
Although glioblastoma (GBM) stem-like cells (GSCs), which retain chemo-radio resistance and recurrence, are key prognostic factors in GBM patients, the molecular mechanisms of GSC development are largely unknown. Recently, several studies revealed that extrinsic ribosome incorporation into somatic cells resulted in stem cell properties and served as a key trigger and factor for the cell reprogramming process. In this study, we aimed to investigate the mechanisms underlying GSCs development by focusing on extrinsic ribosome incorporation into GBM cells. Ribosome-induced cancer cell spheroid (RICCS) formation was significantly upregulated by ribosome incorporation. RICCS showed the stem-like cell characters (number of cell spheroid, stem cell markers, and ability for trans differentiation towards adipocytes and osteocytes). In RICCS, the phosphorylation and protein expression of ribosomal protein S6 (RPS6), an intrinsic ribosomal protein, and STAT3 phosphorylation were upregulated, and involved in the regulation of cell spheroid formation. Consistent with those results, glioma-derived extrinsic ribosome also promoted GBM-RICCS formation through intrinsic RPS6 phosphorylation. Moreover, in glioma patients, RPS6 phosphorylation was dominantly observed in high-grade glioma tissues, and predominantly upregulated in GSCs niches, such as the perinecrosis niche and perivascular niche. Those results indicate the potential biological and clinical significance of extrinsic ribosomal proteins in GSC development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribosomas / Células Madre Neoplásicas / Neoplasias Encefálicas / Glioma Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cells Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribosomas / Células Madre Neoplásicas / Neoplasias Encefálicas / Glioma Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cells Año: 2021 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza