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Curcumin and Radiotherapy Exert Synergistic Anti-Glioma Effect In Vitro.
Zoi, Vasiliki; Galani, Vasiliki; Vartholomatos, Evrysthenis; Zacharopoulou, Natalia; Tsoumeleka, Eftichia; Gkizas, Georgios; Bozios, Georgios; Tsekeris, Pericles; Chousidis, Ieremias; Leonardos, Ioannis; Tzakos, Andreas G; Kyritsis, Athanasios P; Alexiou, George A.
Afiliación
  • Zoi V; Neurosurgical Institute, University of Ioannina, 45500 Ioannina, Greece.
  • Galani V; Department of Anatomy Histology-Embryology, School of Medicine, University of Ioannina, 45110 Ioannina, Greece.
  • Vartholomatos E; Neurosurgical Institute, University of Ioannina, 45500 Ioannina, Greece.
  • Zacharopoulou N; Neurosurgical Institute, University of Ioannina, 45500 Ioannina, Greece.
  • Tsoumeleka E; Neurosurgical Institute, University of Ioannina, 45500 Ioannina, Greece.
  • Gkizas G; Neurosurgical Institute, University of Ioannina, 45500 Ioannina, Greece.
  • Bozios G; Department of Medical Physics, University of Ioannina, 45110 Ioannina, Greece.
  • Tsekeris P; Department of Radiation Oncology, University of Ioannina, 45110 Ioannina, Greece.
  • Chousidis I; Zoology Laboratory, Department of Biological Application and Technology, University of Ioannina, 45110 Ioannina, Greece.
  • Leonardos I; Zoology Laboratory, Department of Biological Application and Technology, University of Ioannina, 45110 Ioannina, Greece.
  • Tzakos AG; Department of Chemistry, Section of Organic Chemistry and Biochemistry, University of Ioannina, 45110 Ioannina, Greece.
  • Kyritsis AP; Neurosurgical Institute, University of Ioannina, 45500 Ioannina, Greece.
  • Alexiou GA; Neurosurgical Institute, University of Ioannina, 45500 Ioannina, Greece.
Biomedicines ; 9(11)2021 Oct 28.
Article en En | MEDLINE | ID: mdl-34829791
Curcumin, a bioactive polyphenol, is known to have anticancer properties. In this study, the effectiveness of curcumin pretreatment as a strategy for radio-sensitizing glioblastoma cell lines was explored. For this, U87 and T98 cells were treated with curcumin, exposed to 2 Gy or 4 Gy of irradiation, and the combined effect was compared to the antiproliferative effect of each agent when given individually. Cell viability and proliferation were evaluated with the trypan blue exclusion assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The synergistic effects of the combination treatment were analyzed with CompuSyn software. To examine how the co-treatment affected different phases of cell-cycle progression, a cell-cycle analysis via flow cytometry was performed. Treatment with curcumin and radiation significantly reduced cell viability in both U87 and T98 cell lines. The combination treatment arrested both cell lines at the G2/M phase to a higher extent than radiation or curcumin treatment alone. The synergistic effect of curcumin when combined with temozolomide resulted in increased tumor cell death. Our results demonstrate for the first time that low doses of curcumin and irradiation exhibit a strong synergistic anti-proliferative effect on glioblastoma cells in vitro. Therefore, this combination may represent an innovative and promising strategy for the treatment of glioblastoma, and further studies are needed to fully understand the molecular mechanism underlying this effect.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomedicines Año: 2021 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomedicines Año: 2021 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Suiza