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Comparative Analysis of BIOCHIP Mosaic-Based Indirect Immunofluorescence with Enzyme-Linked Immunosorbent Assay for Diagnosing Myasthenia Gravis.
Gambino, Caterina Maria; Agnello, Luisa; Lo Sasso, Bruna; Scazzone, Concetta; Giglio, Rosaria Vincenza; Candore, Giuseppina; Ciaccio, Anna Maria; Di Stefano, Vincenzo; Brighina, Filippo; Vidali, Matteo; Ciaccio, Marcello.
Afiliación
  • Gambino CM; Clinical Molecular Medicine and Laboratory Medicine, Institute of Clinical Biochemistry, Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, 90127 Palermo, Italy.
  • Agnello L; Clinical Molecular Medicine and Laboratory Medicine, Institute of Clinical Biochemistry, Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, 90127 Palermo, Italy.
  • Lo Sasso B; Clinical Molecular Medicine and Laboratory Medicine, Institute of Clinical Biochemistry, Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, 90127 Palermo, Italy.
  • Scazzone C; Clinical Molecular Medicine and Laboratory Medicine, Institute of Clinical Biochemistry, Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, 90127 Palermo, Italy.
  • Giglio RV; Clinical Molecular Medicine and Laboratory Medicine, Institute of Clinical Biochemistry, Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, 90127 Palermo, Italy.
  • Candore G; Clinical Molecular Medicine and Laboratory Medicine, Institute of Clinical Biochemistry, Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, 90127 Palermo, Italy.
  • Ciaccio AM; Unit of Clinical Biochemistry, University of Palermo, 90127 Palermo, Italy.
  • Di Stefano V; Unit of Neurology, Department of Biomedicine, Neurosciences and Advanced Diagnostics, 90127 Palermo, Italy.
  • Brighina F; Unit of Neurology, Department of Biomedicine, Neurosciences and Advanced Diagnostics, 90127 Palermo, Italy.
  • Vidali M; Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy.
  • Ciaccio M; Clinical Molecular Medicine and Laboratory Medicine, Institute of Clinical Biochemistry, Department of Biomedicine, Neurosciences and Advanced Diagnostics, University of Palermo, 90127 Palermo, Italy.
Diagnostics (Basel) ; 11(11)2021 Nov 13.
Article en En | MEDLINE | ID: mdl-34829445
BACKGROUND: The detection of anti-acetylcholine receptor (AChR) and anti-muscle-specific tyrosine kinase (MuSK) antibodies is useful in myasthenia gravis (MG) diagnosis and management. BIOCHIP mosaic-based indirect immunofluorescence is a novel analytical method, which employs the simultaneous detection of anti-AChR and anti-MuSK antibodies in a single miniature incubation field. In this study, we compare, for the first time, the BIOCHIP MG mosaic with conventional enzyme-linked immunosorbent assay (ELISA) in the diagnosis of MG. METHODS: A total of 71 patients with MG diagnosis were included in the study. Anti-AChR and anti-MuSK antibodies were measured separately by two different ELISA and simultaneously by BIOCHIP. The results were then compared. RESULTS: The overall concordance between ELISA and BIOCHIP for anti-AChR reactivity was 74%. Cohen's kappa was 0.51 (95% CI 0.32-0.71), which corresponds to 90% of the maximum possible kappa (0.57), given the observed marginal frequencies. The overall concordance for anti-MuSK reactivity was 84%. Cohen's kappa was 0.11 (95% CI 0.00-0.36), which corresponds to 41% of the maximum possible kappa (0.27). CONCLUSION: The overall concordance among assays is not optimal.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Diagnostics (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Diagnostics (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza